Detailed information of TA system TA151268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 179361..179986
• Replicon: plasmid p3R-1
• Accession: NZ_CP049349
• Organism: Escherichia coli strain 3R

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6Q95_RS24020
• Protein ID: WP_000911333.1
• Coordinates: 179588..179986 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: G6Q95_RS24015
• Protein ID: WP_000450520.1
• Coordinates: 179361..179588 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Q95_RS24015	179361..179588	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G6Q95_RS24020	179588..179986	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G6Q95_RS24025	179995..182148	-	2154	WP_000009379.1	type IV conjugative transfer system coupling protein TraD	-
G6Q95_RS24030	182401..183132	-	732	WP_000850416.1	complement resistance protein TraT	-
G6Q95_RS24035	183146..183655	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..194391	194391

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T151268 WP_000911333.1 NZ_CP049349:179588-179986 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T151268 NZ_CP065411:4205718-4205936 [Kosakonia radicincitans]
ATGTCCGAAAAACCATTAACAAAAGTTGATTATTTGATGCGTCTCAGACGTTGTCAGACAATTGATACCCTTGAACGTGT
AATTGAAAAAAATAAATACGAACTTCCGGATGAGGAACTCGCGGTGTTTTATTCAGCAGCCGATCATCGCCTGGCAGAAC
TGACCATGAATAAGCTGTATGACAAAATCCCCTCCTCCGTGTGGAAATTTGTACGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT151268 WP_000450520.1 NZ_CP049349:179361-179588 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT151268 NZ_CP065411:4205320-4205694 [Kosakonia radicincitans]
ATGGACGAATACTCGCCAAAAAGGCATGATATCGCGCAGCTCAAATTTCTCTGCGAAACACTGTACCATGACTGCCTTGT
TAATCTCGAAGAGAGCCATCATGGGTGGGTTAACGATCCTACATCAGCGGTGAACTTGCAGTTAAATGAGCTGATAGAAC
ACATAGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAACAGATAGACGAATATCTT
GACGATACGTTTATGTTGTTCAGTAGCTATGGCATAAATACACATGACTTACAGAAATGGCGCAAAACAGGTAACAAGCT
GTTCCGCTGCTTCGTCAATGTAAGCAAGGCTAATCCTGTTAGCCACTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2