Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 38657..39300 Replicon plasmid p3R-1
Accession NZ_CP049349
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag G6Q95_RS23355 Protein ID WP_001034044.1
Coordinates 38884..39300 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag G6Q95_RS23350 Protein ID WP_001261286.1
Coordinates 38657..38887 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS23335 33794..34024 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
G6Q95_RS23340 34021..34437 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
G6Q95_RS23345 34482..38276 - 3795 WP_001144732.1 hypothetical protein -
G6Q95_RS23350 38657..38887 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G6Q95_RS23355 38884..39300 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
G6Q95_RS23360 39375..40940 + 1566 WP_001128474.1 AAA family ATPase -
G6Q95_RS23365 40925..41947 + 1023 WP_032174242.1 helicase UvrD -
G6Q95_RS23370 42201..42898 - 698 Protein_48 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..194391 194391
- flank IS/Tn - - 42201..42704 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T151264 WP_001034044.1 NZ_CP049349:38884-39300 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T151264 NZ_CP065411:3639054-3639518 [Kosakonia radicincitans]
GTGATTTTTTATCGCCTTGTGTCGCGCCGCTACGCCAGCGAAGCCTGGACCGGCAGCGGTGCGAATCAATATGGCGGCAG
ATGGAATCACAAAGGTCACCCGGCGGTTTATATTGCCTCATCCGTTTCCCTCGCCGCGCTGGAAATGCTGGTGCATGTAC
GCAGCGACACGATCCTTAGCCAGTACGGCCTGTTCAGTATCGACATTCCCGATAAAGAGATTGAATATCTGGATAAACAG
TGGCTGCCGCCGGACTGGCAGGAAAACCCGGCTCCGCTTTCAACGATGGATCTGGGAACCGCCTGGCTGGAAGCCAACAG
CGCCGTCGCGCTGGTAGTTCCTTCCTGCGTCATTCCTCTGGAAAACAATGCCATCCTCAATCCACAGCACGCTGGATTCA
GCAAGTTACTCAAATCAGTAAAAGAGCTTCCTTTTTCGTTTGATGCGCGTCTGGCTTCATCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT151264 WP_001261286.1 NZ_CP049349:38657-38887 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT151264 NZ_CP065411:3638614-3639057 [Kosakonia radicincitans]
ATGAGAACCTGGAGCCCGGAACAAAAACCGGTTGAAAATGCGCTCTGGCGTTTCGCCGGTTTCCCCGCTAACCGCGGTAT
AAAACTTGTTCAGATGTTGAATGAGGGTTTGCCTGTCAGCATTCTGGATAATATCCATGAGTGGACGGAGATGTCTCGCT
CCGACATCCTGCGTGTCACCGGTATTAACGAGCGCAATGTCGCCCGCCGTAAGAGCGCGGGACGCACCTTAACGCCGGAA
GAGAGCGAGCGGATCGCCCGCTTGGTTCGCGTGATCGACGCCGCCGTTCAGTTTTTTGGTGATAAAAAAATGGCGTACGC
CTGGCTGCAAGCGCCGGTACGCGGCCTCGGTAACGTTGCTCCTATCTCCCTGATTGCGACTGAAAGTGGCGCACTGGAAG
TCACCGATCTGATTGGCCGCCTGGAGCACGGAGTATTTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References