Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33794..34437 Replicon plasmid p3R-1
Accession NZ_CP049349
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag G6Q95_RS23340 Protein ID WP_001034046.1
Coordinates 34021..34437 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag G6Q95_RS23335 Protein ID WP_001261278.1
Coordinates 33794..34024 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS23305 29306..29611 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
G6Q95_RS23310 29613..29831 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
G6Q95_RS23315 30397..30909 + 513 WP_000151784.1 hypothetical protein -
G6Q95_RS23320 30943..32076 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
G6Q95_RS23325 32243..33016 - 774 WP_000905949.1 hypothetical protein -
G6Q95_RS23330 33029..33529 - 501 WP_000528932.1 hypothetical protein -
G6Q95_RS23335 33794..34024 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G6Q95_RS23340 34021..34437 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
G6Q95_RS23345 34482..38276 - 3795 WP_001144732.1 hypothetical protein -
G6Q95_RS23350 38657..38887 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
G6Q95_RS23355 38884..39300 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..194391 194391


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T151263 WP_001034046.1 NZ_CP049349:34021-34437 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T151263 NZ_CP065411:c3235744-3235421 [Kosakonia radicincitans]
ATGCTAAAGAGCATTGAAAGCTTTCGAGACCAGTGGCTTAAGGATTTTTTTCTGTATGGTAAGGCTACAGGACACATACC
TAAAGCAATCGAAACGTCTCTTGCTCGAAAGCTAGATATACTAAATGCGGCGACTATAGTTAATGATCTTAGGTCCCCGC
CGGGCAATCGATATGAAAATTTACAGCCGCCACTAAAAGGCTATTCAGCTGTAAGGATAAATGAAAAATATCGATTAATT
TTCATATGGAAAGATGGTAAAGCTACAGATGTTTACCTCGATCCACACAGTTACAAAAACAAAAATAATAACCATCAACT
ATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT151263 WP_001261278.1 NZ_CP049349:33794-34024 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT151263 NZ_CP065411:c3235363-3235028 [Kosakonia radicincitans]
ATGAAACAAGCCATGAGGAAACCAACCACTGTAGGAGATGTGTTACTTTACGAATATCTTGAGCCACTGGATCTCAAAAT
CAACGATCTCGCTGAGATATTGAACGTTCATCGCAACTCCGTGAGTGCTCTGGTGAACAATAATCGTAAACTTACCACAG
ATATGGCTTTCAGATTGTCTGTGGCCTTCGAAACAAGTGTTGAATTCTGGCTTAACTTGCAAAGTAACGTCGATGTTTGG
GAAGTAGAAAATGACGCTCGTACTCAGGAAGAACTCAGTCGCGTAGTAAAAGCGCAGTCCTTTCTGGCCGAAAAAGAACC
TAAAGTAGTAGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References