Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29306..29831 Replicon plasmid p3R-1
Accession NZ_CP049349
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag G6Q95_RS23305 Protein ID WP_001159871.1
Coordinates 29306..29611 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag G6Q95_RS23310 Protein ID WP_000813630.1
Coordinates 29613..29831 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS23290 25262..26467 - 1206 WP_001442122.1 AAA family ATPase -
G6Q95_RS23295 27088..27819 + 732 WP_000504262.1 replication initiation protein -
G6Q95_RS23300 28499..29305 - 807 WP_000016968.1 site-specific integrase -
G6Q95_RS23305 29306..29611 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
G6Q95_RS23310 29613..29831 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
G6Q95_RS23315 30397..30909 + 513 WP_000151784.1 hypothetical protein -
G6Q95_RS23320 30943..32076 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
G6Q95_RS23325 32243..33016 - 774 WP_000905949.1 hypothetical protein -
G6Q95_RS23330 33029..33529 - 501 WP_000528932.1 hypothetical protein -
G6Q95_RS23335 33794..34024 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
G6Q95_RS23340 34021..34437 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..194391 194391


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T151262 WP_001159871.1 NZ_CP049349:c29611-29306 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T151262 NZ_CP065411:2233564-2233666 [Kosakonia radicincitans]
GCCAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT151262 WP_000813630.1 NZ_CP049349:c29831-29613 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT151262 NZ_CP065411:c2233671-2233504 [Kosakonia radicincitans]
CAGATAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTGGCACTTTAAGAATAGATGACGGCGTCAGCTTTTCCAGTCCGCGACAAAAGTGGT
CAGAAAAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References