Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4537106..4537603 Replicon chromosome
Accession NZ_CP049348
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6Q95_RS21445 Protein ID WP_001173343.1
Coordinates 4537430..4537603 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6Q95_RS21440 Protein ID WP_000398619.1
Coordinates 4537106..4537402 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS21420 4533837..4534556 - 720 WP_000611288.1 two-component system response regulator DcuR -
G6Q95_RS21425 4534553..4536184 - 1632 WP_001216487.1 sensor histidine kinase -
G6Q95_RS21430 4536365..4536595 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
G6Q95_RS21435 4536607..4536879 + 273 WP_000405647.1 N-acetyltransferase -
G6Q95_RS21440 4537106..4537402 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6Q95_RS21445 4537430..4537603 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6Q95_RS21450 4537722..4539239 - 1518 WP_001295074.1 lysine--tRNA ligase -
G6Q95_RS21455 4539476..4540933 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T151255 WP_001173343.1 NZ_CP049348:4537430-4537603 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T151255 NZ_CP065410:280549-280731 [Pseudomonas sp. IAC-BECa141]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAGGAGGATGGATGGTATCTGGTCGCCGTCAAAGGCAGTCACCACCAATA
CAAACATCCGGGCAAGCCAGGGAGGGTGACGATCAAGCATCCGGATTCAGATCTTCCCAAGGGAACGATCAACAGCATCT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT151255 WP_000398619.1 NZ_CP049348:4537106-4537402 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT151255 NZ_CP065410:280791-281192 [Pseudomonas sp. IAC-BECa141]
ATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCAGACTACGGAGTGATTATCCCGGATGTACCCGGGTGCTTCTC
CGCAGGCACTACGGTAGCCGAAGCATTTGACAACACTCAGGAGGCACTGGCCTTGCACTATGAAGGACTGGTCGCTGATG
GCGCACCGTTGCCTCGGGTTCAGGACATTGATGCCCATCTCGAGAATCCGGATTACGCCGGCGGTATCTGGGGTGTAGTC
GAGTTTGATATCACTCCCTACTTCGGCAAGTCGGTGCGCTTCAACGCTACGCTCCCGGAGCAGTTGCTGGAGCGTATTGA
CCAGACTGTACGACGTGATCAGCGTTACAGCTCTCGCTCCGGATTTCTGGCTGCTGCTGCCTTGCGTGAATTGTCAGCTT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References