Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3735237..3735868 Replicon chromosome
Accession NZ_CP049348
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag G6Q95_RS17775 Protein ID WP_001259388.1
Coordinates 3735593..3735868 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag G6Q95_RS17770 Protein ID WP_000593555.1
Coordinates 3735237..3735596 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS17745 3731369..3732313 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
G6Q95_RS17750 3732310..3733143 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
G6Q95_RS17755 3733143..3733907 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
G6Q95_RS17760 3733904..3734710 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
G6Q95_RS17765 3734716..3735117 + 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
G6Q95_RS17770 3735237..3735596 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
G6Q95_RS17775 3735593..3735868 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
G6Q95_RS17780 3735941..3737065 - 1125 WP_001615305.1 ABC transporter permease -
G6Q95_RS17785 3737065..3739800 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
G6Q95_RS17790 3739797..3740864 - 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T151252 WP_001259388.1 NZ_CP049348:c3735868-3735593 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T151252 NZ_CP065407:c74772-74395 [Diaphorobacter sp. JS3051]
ATGACCGACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCTACAAGGATCTGATGGCGTTGCCGCCGGACGTGCGTCG
GCGTTTCGGTTACGCGCTGTCGCTGGCGCAGATGGGCGACCAGGACGACGCGGCCAAGGTGCTCAAGGGCTTTGGCGGTG
CCGGTGTGCTGGAAGTCATCGAAGACGATGCCGGTGGCACGTATCGGGCGGTCTACACGGTGAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGGATCGCCACGCCAAAGGTGGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAAGCATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT151252 WP_000593555.1 NZ_CP049348:c3735596-3735237 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT151252 NZ_CP065407:c74414-74079 [Diaphorobacter sp. JS3051]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAACGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCCGATGC
CGAGAAACTGAAAATCAAAACCGGCCTGGTAGTCGAGATCAGGAAAGCGATGCGTGCGCTTGGCTTGACACAACAAGCCG
CGGCCAAGCGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCACGGCGATTTCACCAACCTGTCCGAGCGCAAG
CTGATGGACTGCCTGAATCGCCTCGGTTACGACATCGAAATCAAGGTGCGCCCGGCATCCGAGCCGGTCGGGCACCTGAC
GCTCGCCACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References