Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3048991..3049645 Replicon chromosome
Accession NZ_CP049348
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag G6Q95_RS14335 Protein ID WP_000244772.1
Coordinates 3048991..3049398 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6Q95_RS14340 Protein ID WP_000354046.1
Coordinates 3049379..3049645 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS14315 3044948..3046681 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
G6Q95_RS14320 3046687..3047397 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6Q95_RS14325 3047422..3048318 - 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
G6Q95_RS14330 3048430..3048951 + 522 WP_001055874.1 flavodoxin FldB -
G6Q95_RS14335 3048991..3049398 - 408 WP_000244772.1 protein YgfX Toxin
G6Q95_RS14340 3049379..3049645 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6Q95_RS14345 3049888..3050868 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
G6Q95_RS14350 3051064..3051723 - 660 WP_000250274.1 hemolysin III family protein -
G6Q95_RS14355 3051887..3052198 - 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
G6Q95_RS14360 3052243..3053676 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
G6Q95_RS14365 3053733..3054476 - 744 WP_000951941.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T151248 WP_000244772.1 NZ_CP049348:c3049398-3048991 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T151248 NZ_CP065406:c3586445-3585543 [Diaphorobacter sp. JS3051]
ATGACCGGCAACCTATCCGCGTCGATCCTGGCCCGACTGCTGACCCTCGCCAAACAGCGCGGCGACGACTACAACCTGCT
GCTGAACCGCTACGGCATGGAGCGCCTGCTGGCCCGCGTCAGCACGTCCCCGCATGCTGACCGCTTTCTGCTCAAGGGCG
CCCTGCTCTTTGCGCTGTGGTACGACACCCCGCACCGCCCGACGCGGGACGCTGACTTGCTGGGCTTTGGCCCTGACGAC
GAGGCGAACCTGATCGCCACTTTCCGCGACATCGCCGCCATGGACCTGGGCGACGGCATCGTGTTTGACCCCGAATCCGT
GAAGGCCGACGCCATTCGCGAGGACAACACCTACGGCGGCACCCGCATCACGCTGGTGGCACGCATCGGTTCCGCACGCT
GCGCCCTTCAGATCGACGTGGGCTTCGGCGATGCCGTCACGCCGGGGCCACAGACGGTGGCTTACCCGACTCTGCTGGGT
GACTTCCCAGCCCCCACGCTGCGGGTCTATCCGGTCTACACCGTGATCGCAGAGAAATACCAGGCCATGGTGATGCTGGG
GCAAGCGAACAGCCGCATGAAGGACTTCTTCGACCTGGCGGTGATCGCACGGCGCACGGAACTGGACGGTGCCACACTGG
CAGCAGCCATTGCCGCCACCTTTGCCCGACGCCAGACCGCCCTGCCCACCGGGCGGCCCTTGGCACTGACCCAGCAGTTC
AGCGAAGACGCCGCAAAGCTGCGTCAGTGGCAGGCCTTCCTGAACAAGAACCGCATCGAAGCCGCGAGCCTGGGCGAGAC
CGTCGCACTGCTGGACGACCTGCTGTGGCCACCGACGCAGGTCGCCACCGCCAGCAGTCAGGCAACGGCCACTTGGCGCC
CTGAAGCCCTGCGCTGGGTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT151248 WP_000354046.1 NZ_CP049348:c3049645-3049379 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT151248 NZ_CP065406:c3587041-3586442 [Diaphorobacter sp. JS3051]
ATGAACCAGTCTGACCACATCCTCGAACTGGCCCGGCACCGGCGCGTGCTGCGGGCAGCGGATGTGCGCGAGCATGGCTG
GTCACCGCAGCTGCTGATCCGGCTACACCAGGCCGGAAAGCTGCAACGGCTGGGCCGTGGCCTGTATGGACTGCCCGATG
CTGAAGTCACCGAGCACCAGACGCTGATCGAGGTCTGCCAGCGCGTGCCCAAGGCAGTGCTGTGCCTGCTGAGTGCGCTG
CAATTTCATGAGATCGGCACGCAACTGCCGCATGAAGTGTGGATTGCGCTGCCCGAAGCGACACAGACCCCGGCGCTGAG
CTACCCCACGCTGCGCATCACGCGCCTGCGCGGCACCGCCTACAGCAACGGTATCCAGACCGTGACCGAACACGGTGCCC
CCATCCGCGTGTACAGCGCCGCCAAGACCGTGGCCGACTGCTTCAAGTTCCGCAACAAGATTGGCCTGGACGTGGCGCTG
GAGGCGCTGAAAGATGCATGGCGCAGCCGCAAGGTCACCATAGCCGAACTGAGCCACTTCGCCAAGATCAACCGGGTCGA
CCGTGTCATGCAGCCCTATCTGGAGGCGGTGACGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References