Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2927217..2927800 Replicon chromosome
Accession NZ_CP049348
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag G6Q95_RS13830 Protein ID WP_000254745.1
Coordinates 2927217..2927552 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag G6Q95_RS13835 Protein ID WP_000581937.1
Coordinates 2927552..2927800 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS13815 2923104..2924402 - 1299 WP_000036723.1 phosphopyruvate hydratase -
G6Q95_RS13820 2924490..2926127 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
G6Q95_RS13825 2926355..2927146 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
G6Q95_RS13830 2927217..2927552 - 336 WP_000254745.1 endoribonuclease MazF Toxin
G6Q95_RS13835 2927552..2927800 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
G6Q95_RS13840 2927878..2930112 - 2235 WP_000226815.1 GTP pyrophosphokinase -
G6Q95_RS13845 2930160..2931461 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T151247 WP_000254745.1 NZ_CP049348:c2927552-2927217 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T151247 NZ_CP065405:c547903-547718 [Paraburkholderia caribensis]
ATGAAATACAGCGAGTTCAGGAAGTGGCTGAAGCAGCAAGGCGCGACGTTCGAAAGGCACCGAAGCGGCTCCAGTCACTA
CCGGGTAACGCTCAATGGCAGGACCACCATCTTCCCCGATCACGGTGCGAAGGAAATGGGTAAAGGACTGGTGGAAGCCA
TCAGAAAGCAACTCGGTATCAGGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT151247 WP_000581937.1 NZ_CP049348:c2927800-2927552 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT151247 NZ_CP065405:c547637-547218 [Paraburkholderia caribensis]
ATGCTCAGCTACCCGATTAGCCTGACACCCGACGACAACGGAACGTTGCTCGTCACGTTTCCGGATGTTCCCGAAGCCAT
CACCGTCGGCGACGACGAAGAAGACGCGAAAGTGCAAGCGCTGGATGCGCTCGAGGCCGCGTTCGAAATCTACTTCGCGC
AGAAGCGAAGCATCCCGATGCCGTCAAAGGCCAAACGGGGGCAACCCGTCGTCACGCTGCCGGCGCTCGTCACCGCAAAA
GTGCTGCTCGCGGATGAAATGCTCCGCCAGAACGTACGAAAGGCCGAACTCGCGCGGCGGCTCAGCGTCAACCAGGTGCA
GGTGGATCGACTGCTGAACTTCGGCCATTCATCGAAGATCGAAATGGTCGAGTCGGCGTTTGCGGTATTGGGTAAGCGTC
TTGATGTTCGCGCCGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References