Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1487529..1488167 Replicon chromosome
Accession NZ_CP049348
Organism Escherichia coli strain 3R

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G6Q95_RS06960 Protein ID WP_000813794.1
Coordinates 1487529..1487705 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G6Q95_RS06965 Protein ID WP_001270285.1
Coordinates 1487751..1488167 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Q95_RS06940 1483148..1484362 - 1215 WP_001326689.1 BenE family transporter YdcO -
G6Q95_RS06945 1484415..1484951 + 537 WP_000429153.1 DNA-binding transcriptional regulator SutR -
G6Q95_RS06950 1485024..1486985 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G6Q95_RS06955 1487077..1487307 - 231 WP_000494244.1 YncJ family protein -
G6Q95_RS06960 1487529..1487705 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G6Q95_RS06965 1487751..1488167 + 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G6Q95_RS06970 1488246..1489652 + 1407 WP_000760590.1 PLP-dependent aminotransferase family protein -
G6Q95_RS06975 1489897..1491042 + 1146 WP_000034363.1 ABC transporter substrate-binding protein -
G6Q95_RS06980 1491060..1492073 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
G6Q95_RS06985 1492074..1493015 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1481960..1483108 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T151235 WP_000813794.1 NZ_CP049348:1487529-1487705 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T151235 NZ_CP065401:4008254-4008472 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT151235 WP_001270285.1 NZ_CP049348:1487751-1488167 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT151235 NZ_CP065401:4007853-4008227 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References