Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3493367..3494204 Replicon chromosome
Accession NZ_CP049343
Organism Escherichia coli strain UMB200201_11

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag FM734_RS16700 Protein ID WP_000227784.1
Coordinates 3493662..3494204 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag FM734_RS16695 Protein ID WP_001297137.1
Coordinates 3493367..3493678 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FM734_RS16670 3488387..3489334 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
FM734_RS16675 3489356..3491347 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
FM734_RS16680 3491337..3491951 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
FM734_RS16685 3491951..3492280 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
FM734_RS16690 3492292..3493182 + 891 WP_000971336.1 heme o synthase -
FM734_RS16695 3493367..3493678 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
FM734_RS16700 3493662..3494204 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
FM734_RS16705 3494260..3495195 - 936 WP_001365761.1 sel1 repeat family protein -
FM734_RS16710 3495603..3496967 + 1365 WP_001000975.1 MFS transporter -
FM734_RS16715 3497095..3497586 - 492 WP_001138904.1 nucleotide binding protein YajQ -
FM734_RS16720 3497754..3498665 + 912 WP_000705877.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T151227 WP_000227784.1 NZ_CP049343:3493662-3494204 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T151227 NZ_CP065398:61923-62213 [Bifidobacterium longum]
ATGACAAAGAAATACCAAGACTACGACACCAGCGAATTCCTTGAAACCGAAGACGACATCGCCCGCTACCTCAATGCCGT
AGCAGAATACAATGATCCTGAAATGTTCCAAACCGCACTCGGAAAAGTAGCAAAAGCACGAGGAATGTCCAGCATTGCCA
AAGAGGCGAACGTGTCCAGAGAAAGCCTGTACCGAAGCCTCAGTGCCGAGGGTAATCCCTCGTTCCAAACCATCTCTAAA
GTACTGGCTTCAATGGGGCTTCGACTGACTATACAGCCAGCTGAAGCCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT151227 WP_001297137.1 NZ_CP049343:3493367-3493678 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT151227 NZ_CP065398:61612-61911 [Bifidobacterium longum]
TTGAGATTCAAGAGGACGAGCGAATTCCTTGAATGGCTACGCAAACTAAAAGACAGAGACGCAAAAGCCAAAATCGAAGC
ACACCTTGACTACTGCGAGCTAAAAGGCTCCCTTCTGGGAGATACCAAACCCGTAGGAGACTCCATATACGAGATGCGCT
TTCACTTCGGACCCGGATACCGAATCTACTACGCAAGAAGCGGATCAGAAATCTATCTGCTGCTCATCGGCGGAGATAAA
AGAAAACAACAGGCAGATATCAAAAAAGCCAAGAAACTATTCAGGGAATACTCCGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References