Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3459278..3459896 Replicon chromosome
Accession NZ_CP049343
Organism Escherichia coli strain UMB200201_11

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FM734_RS16530 Protein ID WP_001291435.1
Coordinates 3459678..3459896 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FM734_RS16525 Protein ID WP_000344800.1
Coordinates 3459278..3459652 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FM734_RS16515 3454367..3455560 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FM734_RS16520 3455583..3458732 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FM734_RS16525 3459278..3459652 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FM734_RS16530 3459678..3459896 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FM734_RS16535 3460067..3460618 + 552 WP_000102578.1 maltose O-acetyltransferase -
FM734_RS16540 3460734..3461204 + 471 WP_000136192.1 YlaC family protein -
FM734_RS16545 3461368..3462918 + 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FM734_RS16550 3462960..3463313 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FM734_RS16560 3463692..3464003 + 312 WP_000409911.1 MGMT family protein -
FM734_RS16565 3464034..3464606 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T151226 WP_001291435.1 NZ_CP049343:3459678-3459896 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T151226 NZ_CP065398:61612-61911 [Bifidobacterium longum]
TTGAGATTCAAGAGGACGAGCGAATTCCTTGAATGGCTACGCAAACTAAAAGACAGAGACGCAAAAGCCAAAATCGAAGC
ACACCTTGACTACTGCGAGCTAAAAGGCTCCCTTCTGGGAGATACCAAACCCGTAGGAGACTCCATATACGAGATGCGCT
TTCACTTCGGACCCGGATACCGAATCTACTACGCAAGAAGCGGATCAGAAATCTATCTGCTGCTCATCGGCGGAGATAAA
AGAAAACAACAGGCAGATATCAAAAAAGCCAAGAAACTATTCAGGGAATACTCCGAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT151226 WP_000344800.1 NZ_CP049343:3459278-3459652 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT151226 NZ_CP065398:61923-62213 [Bifidobacterium longum]
ATGACAAAGAAATACCAAGACTACGACACCAGCGAATTCCTTGAAACCGAAGACGACATCGCCCGCTACCTCAATGCCGT
AGCAGAATACAATGATCCTGAAATGTTCCAAACCGCACTCGGAAAAGTAGCAAAAGCACGAGGAATGTCCAGCATTGCCA
AAGAGGCGAACGTGTCCAGAGAAAGCCTGTACCGAAGCCTCAGTGCCGAGGGTAATCCCTCGTTCCAAACCATCTCTAAA
GTACTGGCTTCAATGGGGCTTCGACTGACTATACAGCCAGCTGAAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References