Detailed information of TA system TA151209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1400066..1400985
• Replicon: chromosome
• Accession: NZ_CP049330
• Organism: Bacillus licheniformis strain CP6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: G6536_RS07120
• Protein ID: WP_003180879.1
• Coordinates: 1400239..1400985 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: T5HT37
• Locus tag: G6536_RS07115
• Protein ID: WP_003180877.1
• Coordinates: 1400066..1400239 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6536_RS07095	1395491..1396588	+	1098	WP_003180867.1	mannonate dehydratase	-
G6536_RS07100	1396564..1397412	+	849	WP_009328728.1	SDR family oxidoreductase	-
G6536_RS07105	1397439..1398452	+	1014	WP_003180872.1	zinc-binding alcohol dehydrogenase family protein	-
G6536_RS07110	1398505..1399776	+	1272	WP_011197811.1	MFS transporter	-
G6536_RS07115	1400066..1400239	-	174	WP_003180877.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
G6536_RS07120	1400239..1400985	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
G6536_RS07125	1401096..1402094	-	999	WP_003180881.1	inorganic phosphate transporter	-
G6536_RS07130	1402107..1402724	-	618	WP_003180884.1	DUF47 domain-containing protein	-
G6536_RS07135	1403056..1404813	+	1758	WP_003180886.1	gamma-glutamyltransferase	-
G6536_RS07140	1404942..1405586	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T151209 WP_003180879.1 NZ_CP049330:c1400985-1400239 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T151209 NZ_CP065386:c387328-387032 [Glaesserella parasuis]
ATGGCAGAAAAACACGGTTTACGTCCTTTTGATGTCGCTGAATTTTTGGATAACGAAGAAACAATACAAGCCTATTTAGC
AGAAGTATTAAAAGAGGGCGATCAAGATGAGTTTTTGGAAGCCCTTAATGATGTAGCAAGAGCAAGGGGGATCGCAGAAC
TTGCGAAGCAAACGGGGTTAGGTAGAGAAAGTTTATATAAAACCCTATCTAAAGGAAGTAAACCTCGTTTTGAAACTATT
CAAAAAATTTTAGGAGCATTTAATCTTGTGCTTATCCCACAAATAAAAAATAGTTAA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6945.04 Da
• Isoelectric Point: 10.9186

>AT151209 WP_003180877.1 NZ_CP049330:c1400239-1400066 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT151209 NZ_CP065386:c387671-387336 [Glaesserella parasuis]
ATGTCAGGAATTATCAACCAAATCGTCATTACAGATAATTTTTCAAAGTGGCTAGATGGTTTAAAAAATCCTATTGCTAA
AGCAACAGTGGCAAGAAGAATTAAGAATGCAGTAAAAGGTAATTTTGGTGATCATAAAGCGCTTGCAGGTACTGGTGGAC
TTTATGAAATGCGAATAGCGACAGGCGCTGGTTATCGAATTTACTATGCACAGCAGGGCGATGTTATTTATATTCTGTTA
CAAGGTGGTGATAAATCAACACAGCAAGCCGATATTGAGAAAGCGAAAGCATTGTGGGCAGAATTAAAACAGACAGGAGG
AAGTAATGAATATTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: T5HT37