Detailed information of TA system TA151207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 53920..54504
• Replicon: plasmid pSBC2-5
• Accession: NZ_CP049321
• Organism: Caballeronia sp. SBC2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: SBC2_RS40350
• Protein ID: WP_165107886.1
• Coordinates: 54208..54504 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: SBC2_RS40345
• Protein ID: WP_165107626.1
• Coordinates: 53920..54207 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBC2_RS40330	50324..52009	-	1686	WP_165107620.1	ATP-binding protein	-
SBC2_RS40335	52001..52255	+	255	WP_165107622.1	hypothetical protein	-
SBC2_RS40340	53248..53796	+	549	WP_165107624.1	hypothetical protein	-
SBC2_RS40345	53920..54207	-	288	WP_165107626.1	putative addiction module antidote protein	Antitoxin
SBC2_RS40350	54208..54504	-	297	WP_165107886.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SBC2_RS40355	54624..56657	-	2034	WP_165107629.1	ParB/RepB/Spo0J family partition protein	-
SBC2_RS40360	56869..57291	+	423	WP_165107631.1	antirestriction protein	-
SBC2_RS40365	57630..57914	+	285	WP_165107634.1	helix-turn-helix domain-containing protein	-
SBC2_RS40370	57966..58928	-	963	WP_165107636.1	N-6 DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..195179	195179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (4-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11172.90 Da
• Isoelectric Point: 10.0967

>T151207 WP_165107886.1 NZ_CP049321:c54504-54208 [Caballeronia sp. SBC2]
IFEIKQTETFRLWESKLKDRRARTIIASRLLRFAEGLPGDISPVGNGVSELRIHFGPGYRVYLQQRGTTLVVLLCGGDKS
TQTRDIVLAKKLAQEWEE

Nucleotide

• Length: 297 bp

>T151207 NZ_CP065383:1524104-1524331 [Atribacter laminatus]
ATGACTCCTAAGCTCCCGGTAATTTCTGGTAGGGACTGTGTTAAAGCTCTTGAAAAAATTGGTTATAAAGTGGTAAGACA
AAGAGGCAGTCATTTAAGACTAAAGGATACGAACGGGAAGCTGCTTCCAGTAACAGTGCCAGACCACAAAGAACTACGTC
CGGGGTTAGTTAAAAAGATATTAAACGATGCAAACCTAACTGTGGAAGAATTAATTAAATTATTGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10095.59 Da
• Isoelectric Point: 5.0492

>AT151207 WP_165107626.1 NZ_CP049321:c54207-53920 [Caballeronia sp. SBC2]
MAEKLTTYDPAAALVDSEEIAFFMADALETGDAAYIAKALGVVARAKGMTQIAKDTGLSREQLYRSFSETGNPTLKTTLA
VMRALGIDLTAKVHA

Nucleotide

• Length: 288 bp

>AT151207 NZ_CP065383:1523907-1524107 [Atribacter laminatus]
GTGAGAACTTACCCTGTTATTATTGAGCAGGATGACACTGGCTATGTTGTATCATGTCCTTCTTTTCATGGATGTTATTC
TCAAGGAGATACGATAGATGAAGCCCTGAAAAACATAAAAGAAGCAATTGAATTATGCCTTGACGATGAAGAAATTCCAA
TTGGCAGTGTTATAGTTGGTAATGTGGTGTTAGAAAGATGA