151194

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 4780..5368 Replicon plasmid pEx003
Accession NZ_CP049315
Organism Acinetobacter baumannii strain Ex003

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag G5571_RS18760 Protein ID WP_000438826.1
Coordinates 4780..5067 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A335NKQ9
Locus tag G5571_RS18765 Protein ID WP_024434403.1
Coordinates 5054..5368 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5571_RS18740 (G5571_p00001) 145..1074 + 930 WP_000845850.1 replication initiation protein RepM -
G5571_RS18745 (G5571_p00002) 1141..1686 + 546 WP_000736776.1 plasmid replication DNA-binding protein -
G5571_RS18750 (G5571_p00003) 1917..2768 - 852 WP_004870777.1 universal stress protein -
G5571_RS18755 (G5571_p00004) 2781..4268 - 1488 WP_000953323.1 SulP family inorganic anion transporter -
G5571_RS18760 (G5571_p00005) 4780..5067 + 288 WP_000438826.1 BrnT family toxin Toxin
G5571_RS18765 (G5571_p00006) 5054..5368 + 315 WP_024434403.1 BrnA antitoxin family protein Antitoxin
G5571_RS18770 (G5571_p00007) 5496..7907 + 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
G5571_RS18775 (G5571_p00008) 8212..8673 + 462 WP_000761345.1 DIP1984 family protein -
G5571_RS18780 (G5571_p00009) 9022..9477 - 456 WP_000932329.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11844 11844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T151194 WP_000438826.1 NZ_CP049315:4780-5067 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T151194 NZ_CP065369:c717713-717435 [Vibrio parahaemolyticus]
ATGATTTTGTGGGAAGAAGAGTCTCTCAACGATCGCGAAAAGATCTTTGAGTTTCTCTACGACTTTAACCCCGAAGCTGC
TGACAAAACCGACGATATTATTGAAGCAAAAGTTGAAAACCTTTTAGAGCAACCTCATATGGGAGTCCAGCGTGACGGAA
TTAGAGGGCGATTGCTCATTATTCCTGAGATTTCGATGATCGTTTCCTACTGGGTTGAAGGCGACATCATTCGCATTATG
CGTGTGTTACACCAAAAACAAAAATTCCCAACGGACTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12222.86 Da        Isoelectric Point: 7.2026

>AT151194 WP_024434403.1 NZ_CP049315:5054-5368 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTIPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT151194 NZ_CP065369:c717994-717710 [Vibrio parahaemolyticus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAGCGACTAGCCCAACAAATGGCCGAAAGCCAAGGTAGAAC
GCTAAGTGATGCTTGTCGCGAACTGACTGAGCAACTAGCTGACCAACAGAGAAAGACTTTATCTCACGACGCATGGTTAA
CTGAACAAGTTAACTTAGCTTTTGAGAAGTTTGACTCAGGAAAAGCCACTTTTGTTGACCATGAGTCAGCTAAATCTCGT
ATGGCCGAGCGTAAAGCTAAAATCCGTAACCGAGGCAAATTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB A0A335NKQ9

References