Detailed information of TA system TA151190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 2811349..2811985
• Replicon: chromosome
• Accession: NZ_CP049296
• Organism: Priestia megaterium strain S188

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: G5C61_RS15130
• Protein ID: WP_013055004.1
• Coordinates: 2811635..2811985 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: G5C61_RS15125
• Protein ID: WP_013055003.1
• Coordinates: 2811349..2811630 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C61_RS15100	2806715..2807686	+	972	WP_164796846.1	UV DNA damage repair endonuclease UvsE	-
G5C61_RS15105	2807695..2808297	-	603	WP_164796847.1	rhomboid family intramembrane serine protease	-
G5C61_RS15110	2808362..2808727	+	366	WP_164798314.1	holo-ACP synthase	-
G5C61_RS15115	2808786..2809844	+	1059	WP_057243271.1	outer membrane lipoprotein carrier protein LolA	-
G5C61_RS15120	2809958..2811148	+	1191	WP_164796848.1	alanine racemase	-
G5C61_RS15125	2811349..2811630	+	282	WP_013055003.1	hypothetical protein	Antitoxin
G5C61_RS15130	2811635..2811985	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
G5C61_RS15135	2812145..2812981	+	837	WP_013055005.1	STAS domain-containing protein	-
G5C61_RS15140	2812984..2813340	+	357	WP_013055006.1	STAS domain-containing protein	-
G5C61_RS15145	2813344..2813745	+	402	WP_013055007.1	anti-sigma regulatory factor	-
G5C61_RS15150	2813759..2814769	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
G5C61_RS15155	2814829..2815161	+	333	WP_013055009.1	anti-sigma factor antagonist	-
G5C61_RS15160	2815158..2815643	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
G5C61_RS15165	2815609..2816403	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T151190 WP_013055004.1 NZ_CP049296:2811635-2811985 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T151190 NZ_CP065366:c1503259-1502969 [Vibrio cyclitrophicus]
ATGACCTATAAACTCGACTTTAAAAAGAGCGCACTCAAAGAGTGGAAAAAGCTTGGCTCAACGCTTCAACAACAATTTAA
GAAAAAGCTAATCGAGCGCTTAGATAACCCGCATGTTCCGGCTTCAAAACTCTCTGGAGCTGACAACATGTATAAGATTA
AGCTTCGCCAATCGGGCTACCGTCTCGTCTACAGAGTTGAAGACGATGTCATCATTGTAACCGTCTTAGCAGTCGGAAAA
CGCGAACGTAGCGATGTTTACCGTAAAGCTATGAAAAGGTTAGATGATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT151190 WP_013055003.1 NZ_CP049296:2811349-2811630 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT151190 NZ_CP065366:c1503497-1503249 [Vibrio cyclitrophicus]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACTGAATTAAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTACGGTGAACCTGTTGCTGTACTGAACCGAAATGAGCCAGCATTTTATTGCGTACCAGCCGAAGCGTACGAAATGATGA
TGGACAGACTCGAAGATCTTGAACTACTAACTATTGCGAAAGAGCGTGAATCTGAAGAGAGCATTTCGGTAAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9