Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3123304..3124043 Replicon chromosome
Accession NZ_CP049290
Organism Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Toxin (Protein)


Gene name tacT Uniprot ID A0A822W909
Locus tag G6324_RS15130 Protein ID WP_017382345.1
Coordinates 3123304..3123789 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag G6324_RS15135 Protein ID WP_003857131.1
Coordinates 3123777..3124043 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6324_RS15110 (G6324_15115) 3119423..3120037 + 615 WP_023303743.1 NUDIX hydrolase -
G6324_RS15115 (G6324_15120) 3120237..3120869 + 633 WP_001567369.1 hypothetical protein -
G6324_RS15120 (G6324_15125) 3120898..3122301 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
G6324_RS15125 (G6324_15130) 3122684..3123253 + 570 WP_017382346.1 hypothetical protein -
G6324_RS15130 (G6324_15135) 3123304..3123789 - 486 WP_017382345.1 GNAT family N-acetyltransferase Toxin
G6324_RS15135 (G6324_15140) 3123777..3124043 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
G6324_RS15140 (G6324_15145) 3124107..3125036 - 930 WP_023303744.1 LysR family transcriptional regulator -
G6324_RS15145 (G6324_15150) 3125166..3126554 + 1389 WP_032619651.1 MFS transporter -
G6324_RS15150 (G6324_15155) 3126576..3127571 - 996 WP_023303746.1 DUF2891 domain-containing protein -
G6324_RS15155 (G6324_15160) 3127581..3128567 - 987 WP_017382341.1 DUF979 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3120898..3122301 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17510.21 Da        Isoelectric Point: 9.9658

>T151176 WP_017382345.1 NZ_CP049290:c3123789-3123304 [Enterobacter hormaechei subsp. steigerwaltii]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARAFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T151176 NZ_CP065354:c2073661-2073452 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT151176 WP_003857131.1 NZ_CP049290:c3124043-3123777 [Enterobacter hormaechei subsp. steigerwaltii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT151176 NZ_CP065354:c2073966-2073673 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822W909

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References