Detailed information of TA system TA151173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 640282..640898
• Replicon: chromosome
• Accession: NZ_CP049290
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6324_RS03050
• Protein ID: WP_015569913.1
• Coordinates: 640282..640653 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: G6324_RS03055
• Protein ID: WP_015569912.1
• Coordinates: 640656..640898 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6324_RS03035 (G6324_03030)	637782..638684	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
G6324_RS03040 (G6324_03035)	638681..639316	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
G6324_RS03045 (G6324_03040)	639313..640242	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
G6324_RS03050 (G6324_03045)	640282..640653	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
G6324_RS03055 (G6324_03050)	640656..640898	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
G6324_RS03060 (G6324_03055)	641097..642017	+	921	WP_015569911.1	alpha/beta hydrolase	-
G6324_RS03065 (G6324_03060)	642026..642967	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
G6324_RS03070 (G6324_03065)	643012..643449	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
G6324_RS03075 (G6324_03070)	643446..644327	-	882	WP_003861949.1	virulence factor BrkB family protein	-
G6324_RS03080 (G6324_03075)	644321..644920	-	600	WP_003861947.1	glucose-1-phosphatase	-
G6324_RS03085 (G6324_03080)	645039..645839	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T151173 WP_015569913.1 NZ_CP049290:c640653-640282 [Enterobacter hormaechei subsp. steigerwaltii]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T151173 NZ_CP065354:c1864506-1864255 [Staphylococcus aureus]
ATGAATAACCGTGAACAAATAGAACAATCAGTGATCAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGGTTGCTAAA
AGAGATTGAGGAAGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTAAGGGTTTACATAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGAGTCAAGTTGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT151173 WP_015569912.1 NZ_CP049290:c640898-640656 [Enterobacter hormaechei subsp. steigerwaltii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT151173 NZ_CP065354:c1864265-1864104 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAACTT
AA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3