Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 110046..110671 Replicon plasmid p602068_185
Accession NZ_CP049286
Organism Shigella boydii strain 602068

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag G5S54_RS23715 Protein ID WP_000911318.1
Coordinates 110273..110671 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag G5S54_RS23710 Protein ID WP_000450530.1
Coordinates 110046..110273 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S54_RS23710 110046..110273 + 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
G5S54_RS23715 110273..110671 + 399 WP_000911318.1 tRNA(fMet)-specific endonuclease VapC Toxin
G5S54_RS23720 110680..111162 - 483 Protein_130 conjugal transfer protein TraD -
G5S54_RS23725 111218..111915 + 698 Protein_131 IS1 family transposase -
G5S54_RS23730 111931..112602 + 672 Protein_132 IS630 family transposase -
G5S54_RS23735 112756..113700 - 945 WP_005027201.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
G5S54_RS23740 113765..114715 - 951 WP_005059240.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC3 / ospI / ospE2 / senB / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH1.4 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospC2 / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ 1..185316 185316
- inside IScluster/Tn - ipaH9.8 111412..130928 19516


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T151170 WP_000911318.1 NZ_CP049286:110273-110671 [Shigella boydii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T151170 NZ_CP065353:c2396933-2396667 [Staphylococcus aureus]
ATGGATAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGCAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGCGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATAAAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT151170 WP_000450530.1 NZ_CP049286:110046-110273 [Shigella boydii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT151170 NZ_CP065353:c2397184-2396933 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References