Detailed information of TA system TA151169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 90503..91278
• Replicon: plasmid p602068_185
• Accession: NZ_CP049286
• Organism: Shigella boydii strain 602068

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q31SE5
• Locus tag: G5S54_RS23605
• Protein ID: WP_000405246.1
• Coordinates: 90503..90985 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: G5S54_RS23610
• Protein ID: WP_010921625.1
• Coordinates: 90976..91278 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S54_RS23575	86946..87083	+	138	WP_011378983.1	hypothetical protein	-
G5S54_RS23580	87365..87517	-	153	Protein_102	IS3 family transposase	-
G5S54_RS23585	87573..88265	+	693	WP_039080395.1	type III secretion system effector cysteine methyltransferase OspZ	-
G5S54_RS23595	89531..89869	+	339	Protein_105	transposase	-
G5S54_RS23600	89912..90435	-	524	Protein_106	transposase	-
G5S54_RS23605	90503..90985	-	483	WP_000405246.1	GNAT family N-acetyltransferase	Toxin
G5S54_RS23610	90976..91278	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
G5S54_RS23615	91550..91822	+	273	WP_000019158.1	hypothetical protein	-
G5S54_RS23620	91803..93011	+	1209	WP_000198550.1	IS91 family transposase	-
G5S54_RS23625	93150..94604	-	1455	WP_000701107.1	type 3 secretion system effector OspC2	-
G5S54_RS23630	95029..95873	+	845	WP_114142280.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospC3 / ospI / ospE2 / senB / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH1.4 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospC2 / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ	1..185316	185316
	-	inside	IScluster/Tn	-	ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospC2	57994..98213	40219

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17748.55 Da
• Isoelectric Point: 7.0382

>T151169 WP_000405246.1 NZ_CP049286:c90985-90503 [Shigella boydii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCIQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T151169 NZ_CP065353:c2075043-2074834 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACATCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT151169 WP_010921625.1 NZ_CP049286:c91278-90976 [Shigella boydii]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT151169 NZ_CP065353:c2075348-2075055 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: Q31SE5

Antitoxin

• AlphaFold DB: Q7BEG8