Detailed information of TA system TA151168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 52090..52847
• Replicon: plasmid p602068_185
• Accession: NZ_CP049286
• Organism: Shigella boydii strain 602068

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q31SN7
• Locus tag: G5S54_RS23360
• Protein ID: WP_000501969.1
• Coordinates: 52362..52847 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q31SN8
• Locus tag: G5S54_RS23355
• Protein ID: WP_011114751.1
• Coordinates: 52090..52374 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S54_RS23335	47414..47785	+	372	Protein_53	ATP-binding protein	-
G5S54_RS23340	47778..48566	-	789	WP_005116773.1	AraC family invasion system transcriptional regulator VirF	-
G5S54_RS23345	49065..50191	+	1127	Protein_55	IS110-like element ISSso6 family transposase	-
G5S54_RS23355	52090..52374	+	285	WP_011114751.1	DUF1778 domain-containing protein	Antitoxin
G5S54_RS23360	52362..52847	+	486	WP_000501969.1	GNAT family N-acetyltransferase	Toxin
G5S54_RS23365	52881..54059	+	1179	WP_044325982.1	group II intron reverse transcriptase/maturase	-
G5S54_RS23370	54142..54296	+	155	Protein_60	transposase domain-containing protein	-
G5S54_RS23375	54350..54832	-	483	Protein_61	IS110 family transposase	-
G5S54_RS23380	55088..56068	-	981	WP_000431558.1	ParB/RepB/Spo0J family partition protein	-
G5S54_RS23385	56068..57267	-	1200	WP_000200287.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospC3 / ospI / ospE2 / senB / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH1.4 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospC2 / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ	1..185316	185316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17712.54 Da
• Isoelectric Point: 10.1083

>T151168 WP_000501969.1 NZ_CP049286:52362-52847 [Shigella boydii]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T151168 NZ_CP065353:c2055449-2055087 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10871.52 Da
• Isoelectric Point: 4.6812

>AT151168 WP_011114751.1 NZ_CP049286:52090-52374 [Shigella boydii]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT151168 NZ_CP065353:c2055616-2055446 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: Q31SN7

Antitoxin

• AlphaFold DB: A0A4V1CUJ1