Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3318238..3318821 Replicon chromosome
Accession NZ_CP049285
Organism Shigella boydii strain 602068

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag G5S54_RS16815 Protein ID WP_000254733.1
Coordinates 3318238..3318573 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag G5S54_RS16820 Protein ID WP_000581937.1
Coordinates 3318573..3318821 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S54_RS16800 3314125..3315423 - 1299 WP_000036723.1 phosphopyruvate hydratase -
G5S54_RS16805 3315511..3317148 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
G5S54_RS16810 3317376..3318167 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
G5S54_RS16815 3318238..3318573 - 336 WP_000254733.1 endoribonuclease MazF Toxin
G5S54_RS16820 3318573..3318821 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
G5S54_RS16825 3318899..3321133 - 2235 WP_000226815.1 GTP pyrophosphokinase -
G5S54_RS16830 3321181..3322482 - 1302 WP_000046825.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T151164 WP_000254733.1 NZ_CP049285:c3318573-3318238 [Shigella boydii]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T151164 NZ_CP065352:c8097-7792 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT151164 WP_000581937.1 NZ_CP049285:c3318821-3318573 [Shigella boydii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT151164 NZ_CP065352:c8317-8099 [Klebsiella pneumoniae]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References