Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1765283..1765858 Replicon chromosome
Accession NZ_CP049285
Organism Shigella boydii strain 602068

Toxin (Protein)


Gene name pemK Uniprot ID I6EL31
Locus tag G5S54_RS08875 Protein ID WP_000373726.1
Coordinates 1765541..1765858 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID I6EL32
Locus tag G5S54_RS08870 Protein ID WP_000288814.1
Coordinates 1765283..1765540 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S54_RS08840 1760349..1761517 + 1169 WP_178947416.1 IS3 family transposase -
G5S54_RS08845 1761680..1761889 + 210 WP_001044499.1 hypothetical protein -
G5S54_RS08850 1761917..1762282 + 366 WP_000253098.1 hypothetical protein -
G5S54_RS08855 1762295..1763899 + 1605 WP_000628841.1 hypothetical protein -
G5S54_RS08860 1764270..1764731 + 462 WP_000201268.1 hypothetical protein -
G5S54_RS08865 1764728..1764934 + 207 WP_000979502.1 helix-turn-helix domain-containing protein -
G5S54_RS08870 1765283..1765540 + 258 WP_000288814.1 antitoxin Antitoxin
G5S54_RS08875 1765541..1765858 + 318 WP_000373726.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
G5S54_RS08880 1766723..1767127 + 405 Protein_1723 DNA-binding protein -
G5S54_RS08885 1767180..1768393 + 1214 WP_134810244.1 IS3 family transposase -
G5S54_RS08890 1768436..1768921 + 486 Protein_1725 integrase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1750732..1782343 31611


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-105)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11293.94 Da        Isoelectric Point: 10.2965

>T151155 WP_000373726.1 NZ_CP049285:1765541-1765858 [Shigella boydii]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARL

Download         Length: 318 bp

>T151155 NZ_CP065351:800925-801335 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9280.76 Da        Isoelectric Point: 4.5821

>AT151155 WP_000288814.1 NZ_CP049285:1765283-1765540 [Shigella boydii]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT151155 NZ_CP065351:800678-800944 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T2UX62

Antitoxin

Source ID Structure
AlphaFold DB A0A085HLS8

References