Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 71263..72020 Replicon plasmid p600657_208
Accession NZ_CP049282
Organism Shigella boydii strain 600657

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag G5S55_RS24065 Protein ID WP_000501969.1
Coordinates 71263..71748 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag G5S55_RS24070 Protein ID WP_011114751.1
Coordinates 71736..72020 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S55_RS24035 66758..66937 - 180 Protein_82 transposase -
G5S55_RS24040 66974..68183 - 1210 Protein_83 IS3 family transposase -
G5S55_RS24045 68485..68781 + 297 WP_039059935.1 helix-turn-helix domain-containing protein -
G5S55_RS24050 68833..69252 + 420 WP_000435428.1 IS66 family insertion sequence hypothetical protein -
G5S55_RS24055 69249..69599 + 351 Protein_86 IS66 family insertion sequence element accessory protein TnpB -
G5S55_RS24060 69630..71222 + 1593 WP_178930521.1 IS66 family transposase -
G5S55_RS24065 71263..71748 - 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
G5S55_RS24070 71736..72020 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
G5S55_RS24075 72693..73861 + 1169 WP_134802537.1 IS3 family transposase -
G5S55_RS24080 73871..73997 - 127 Protein_91 IS110 family transposase -
G5S55_RS24090 75561..75830 - 270 Protein_93 transposase -
G5S55_RS24095 75945..76583 - 639 WP_000607008.1 type III secretion system effector OspI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 / ospD3/senA / ospC1 / senB / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ipaH9.8 / ospG 1..208836 208836
- inside IScluster/Tn - ospI / ipaH1.4 / ospE2 / ospD3/senA / ospC1 66749..100649 33900


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T151152 WP_000501969.1 NZ_CP049282:c71748-71263 [Shigella boydii]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T151152 NZ_CP065347:c5037677-5037309 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT151152 WP_011114751.1 NZ_CP049282:c72020-71736 [Shigella boydii]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT151152 NZ_CP065347:c5037895-5037674 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References