Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 195421..195980 Replicon plasmid p600690_216
Accession NZ_CP049279
Organism Shigella boydii strain 600690

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag G5S56_RS24780 Protein ID WP_000421262.1
Coordinates 195421..195696 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q327A8
Locus tag G5S56_RS24785 Protein ID WP_001178088.1
Coordinates 195696..195980 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S56_RS24745 (G5S56_24745) 191831..191938 - 108 WP_023592908.1 transposase domain-containing protein -
G5S56_RS24750 (G5S56_24750) 191962..192267 - 306 WP_078167349.1 type II toxin-antitoxin system toxin CcdB -
G5S56_RS24755 (G5S56_24755) 192269..192487 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA -
G5S56_RS24760 (G5S56_24760) 193023..194114 - 1092 Protein_233 IS66 family transposase -
G5S56_RS24765 (G5S56_24765) 194122..194690 + 569 Protein_234 plasmid segregation protein ParM -
G5S56_RS25570 194693..194884 + 192 WP_284522470.1 plasmid partitioning/stability family protein -
G5S56_RS24775 (G5S56_24775) 195152..195289 + 138 WP_171700985.1 hypothetical protein -
G5S56_RS24780 (G5S56_24780) 195421..195696 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G5S56_RS24785 (G5S56_24785) 195696..195980 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein Antitoxin
G5S56_RS24790 (G5S56_24790) 196432..196683 + 252 WP_000433928.1 transporter -
G5S56_RS24795 (G5S56_24795) 196733..196942 + 210 Protein_240 peptidoglycan-binding protein -
G5S56_RS24800 (G5S56_24800) 197042..197227 - 186 Protein_241 ATP-binding protein -
G5S56_RS24805 (G5S56_24805) 197563..198033 + 471 Protein_242 IS1 family transposase -
G5S56_RS24810 (G5S56_24810) 198192..198830 + 639 WP_000607008.1 type III secretion system effector OspI -
G5S56_RS24815 (G5S56_24815) 198945..199214 + 270 Protein_244 transposase -
G5S56_RS24820 (G5S56_24820) 199302..199598 + 297 Protein_245 IS3 family transposase -
G5S56_RS24825 (G5S56_24825) 199613..200959 - 1347 WP_000483770.1 IS4-like element IS4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / ospE2 / ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC 1..216073 216073
- inside IScluster/Tn - ospG / ipaH9.8 / ospI 186448..215420 28972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T151132 WP_000421262.1 NZ_CP049279:c195696-195421 [Shigella boydii]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T151132 NZ_CP065343:559361-559645 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10497.93 Da        Isoelectric Point: 6.2150

>AT151132 WP_001178088.1 NZ_CP049279:c195980-195696 [Shigella boydii]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Download         Length: 285 bp

>AT151132 NZ_CP065343:559129-559371 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB Q327A8

References