Detailed information of TA system TA151131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 191962..192487
• Replicon: plasmid p600690_216
• Accession: NZ_CP049279
• Organism: Shigella boydii strain 600690

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: G5S56_RS24750
• Protein ID: WP_078167349.1
• Coordinates: 191962..192267 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q7BEK0
• Locus tag: G5S56_RS24755
• Protein ID: WP_000813626.1
• Coordinates: 192269..192487 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S56_RS24720 (G5S56_24720)	187990..188256	-	267	Protein_225	IS66 family insertion sequence element accessory protein TnpB	-
G5S56_RS24725 (G5S56_24725)	188613..189203	-	591	WP_000705601.1	type III secretion system effector protein kinase OspG	-
G5S56_RS24730 (G5S56_24730)	189286..189455	-	170	Protein_227	type II toxin-antitoxin system toxin YacB	-
G5S56_RS24735 (G5S56_24735)	189455..189724	-	270	WP_000079956.1	type II toxin-antitoxin system antitoxin YacA	-
G5S56_RS24740 (G5S56_24740)	189932..191569	-	1638	WP_000936808.1	T3SS effector E3 ubiquitin-protein ligase IpaH9.8	-
G5S56_RS24745 (G5S56_24745)	191831..191938	-	108	WP_023592908.1	transposase domain-containing protein	-
G5S56_RS24750 (G5S56_24750)	191962..192267	-	306	WP_078167349.1	type II toxin-antitoxin system toxin CcdB	Toxin
G5S56_RS24755 (G5S56_24755)	192269..192487	-	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
G5S56_RS24760 (G5S56_24760)	193023..194114	-	1092	Protein_233	IS66 family transposase	-
G5S56_RS24765 (G5S56_24765)	194122..194690	+	569	Protein_234	plasmid segregation protein ParM	-
G5S56_RS25570	194693..194884	+	192	WP_284522470.1	plasmid partitioning/stability family protein	-
G5S56_RS24775 (G5S56_24775)	195152..195289	+	138	WP_171700985.1	hypothetical protein	-
G5S56_RS24780 (G5S56_24780)	195421..195696	-	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G5S56_RS24785 (G5S56_24785)	195696..195980	-	285	WP_001178088.1	ribbon-helix-helix domain-containing protein	-
G5S56_RS24790 (G5S56_24790)	196432..196683	+	252	WP_000433928.1	transporter	-
G5S56_RS24795 (G5S56_24795)	196733..196942	+	210	Protein_240	peptidoglycan-binding protein	-
G5S56_RS24800 (G5S56_24800)	197042..197227	-	186	Protein_241	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / ospE2 / ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC	1..216073	216073
	-	inside	IScluster/Tn	-	ospG / ipaH9.8 / ospI	186448..215420	28972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11732.64 Da
• Isoelectric Point: 6.4674

>T151131 WP_078167349.1 NZ_CP049279:c192267-191962 [Shigella boydii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLAGARLLLDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T151131 NZ_CP065343:402546-403463 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8333.36 Da
• Isoelectric Point: 6.2838

>AT151131 WP_000813626.1 NZ_CP049279:c192487-192269 [Shigella boydii]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT151131 NZ_CP065343:401782-402540 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: Q7BEK0