Detailed information of TA system TA151130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 176336..176961
• Replicon: plasmid p600690_216
• Accession: NZ_CP049279
• Organism: Shigella boydii strain 600690

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G5S56_RS24655
• Protein ID: WP_078167309.1
• Coordinates: 176563..176961 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: G5S56_RS24650
• Protein ID: WP_000450530.1
• Coordinates: 176336..176563 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S56_RS24650 (G5S56_24650)	176336..176563	+	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G5S56_RS24655 (G5S56_24655)	176563..176961	+	399	WP_078167309.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G5S56_RS24660 (G5S56_24660)	176972..177184	-	213	Protein_214	DUF932 domain-containing protein	-
G5S56_RS24665 (G5S56_24665)	177303..177589	-	287	Protein_215	hypothetical protein	-
G5S56_RS24670 (G5S56_24670)	177569..178579	+	1011	WP_178966746.1	IS630 family transposase	-
G5S56_RS24675 (G5S56_24675)	178733..179676	-	944	Protein_217	lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase	-
G5S56_RS24680 (G5S56_24680)	179741..180691	-	951	WP_004994053.1	virulence factor VirK	-
G5S56_RS24685 (G5S56_24685)	180696..181784	-	1089	WP_005027207.1	putative hexosyltransferase CapU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / ospE2 / ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC	1..216073	216073
	-	flank	IS/Tn	-	-	177653..178579	926

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14887.17 Da
• Isoelectric Point: 8.5264

>T151130 WP_078167309.1 NZ_CP049279:176563-176961 [Shigella boydii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVVGLRTEDWS

Nucleotide

• Length: 399 bp

>T151130 NZ_CP065343:79039-79422 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT151130 WP_000450530.1 NZ_CP049279:176336-176563 [Shigella boydii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT151130 NZ_CP065343:78797-79039 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: Q31SG2