Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 150008..150783 Replicon plasmid p600690_216
Accession NZ_CP049279
Organism Shigella boydii strain 600690

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag G5S56_RS24530 Protein ID WP_000405248.1
Coordinates 150008..150490 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag G5S56_RS24535 Protein ID WP_010921625.1
Coordinates 150481..150783 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S56_RS24500 (G5S56_24500) 146451..146588 + 138 WP_011378983.1 hypothetical protein -
G5S56_RS24505 (G5S56_24505) 146870..147022 - 153 Protein_181 IS3 family transposase -
G5S56_RS24510 (G5S56_24510) 147078..147770 + 693 WP_078167358.1 type III secretion system effector cysteine methyltransferase OspZ -
G5S56_RS24520 (G5S56_24520) 149036..149374 + 339 Protein_184 integrase core domain-containing protein -
G5S56_RS24525 (G5S56_24525) 149417..149940 - 524 Protein_185 IS3-like element ISSfl11 family transposase -
G5S56_RS24530 (G5S56_24530) 150008..150490 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
G5S56_RS24535 (G5S56_24535) 150481..150783 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
G5S56_RS24540 (G5S56_24540) 151055..151327 + 273 WP_000019158.1 hypothetical protein -
G5S56_RS24545 (G5S56_24545) 151308..152435 + 1128 WP_178966740.1 transposase -
G5S56_RS24550 (G5S56_24550) 152607..152735 + 129 Protein_190 IS5/IS1182 family transposase -
G5S56_RS24555 (G5S56_24555) 152884..154338 - 1455 WP_000701110.1 type 3 secretion system effector OspC2 -
G5S56_RS24560 (G5S56_24560) 154763..155607 + 845 WP_178966742.1 IS5-like element ISSfl7 family transposase -
G5S56_RS25560 155702..155779 + 78 Protein_193 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / ospE2 / ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospI / papC 1..216073 216073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T151129 WP_000405248.1 NZ_CP049279:c150490-150008 [Shigella boydii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T151129 NZ_CP065342:c106848-106318 [Klebsiella pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT151129 WP_010921625.1 NZ_CP049279:c150783-150481 [Shigella boydii]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT151129 NZ_CP065342:c107121-106852 [Klebsiella pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References