Detailed information of TA system TA151111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1639834..1640423
• Replicon: chromosome
• Accession: NZ_CP049278
• Organism: Shigella boydii strain 600690

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A398TMA1
• Locus tag: G5S56_RS07855
• Protein ID: WP_000513533.1
• Coordinates: 1639834..1640082 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G5S56_RS07860
• Protein ID: WP_001016018.1
• Coordinates: 1640079..1640423 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S56_RS07825 (1635108)	1635108..1636049	-	942	Protein_1523	tyrosine-type recombinase/integrase	-
G5S56_RS07830 (1636111)	1636111..1636344	+	234	Protein_1524	transposase	-
G5S56_RS07840 (1637596)	1637596..1637691	-	96	WP_284522451.1	host nuclease inhibitor GamL	-
G5S56_RS07845 (1637712)	1637712..1637993	-	282	WP_000934193.1	hypothetical protein	-
G5S56_RS07850 (1638288)	1638288..1639178	-	891	WP_078167383.1	Rha family phage regulatory protein	-
G5S56_RS07855 (1639834)	1639834..1640082	+	249	WP_000513533.1	type II toxin-antitoxin system HicA family toxin	Toxin
G5S56_RS07860 (1640079)	1640079..1640423	+	345	WP_001016018.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
G5S56_RS07865 (1640538)	1640538..1640708	-	171	WP_164834428.1	hypothetical protein	-
G5S56_RS07870 (1640830)	1640830..1641549	-	720	WP_000162429.1	LexA family transcriptional regulator	-
G5S56_RS07875 (1641655)	1641655..1641894	+	240	WP_000171144.1	helix-turn-helix transcriptional regulator	-
G5S56_RS25125 (1641941)	1641941..1642198	+	258	Protein_1534	hypothetical protein	-
G5S56_RS07885 (1642420)	1642420..1642626	+	207	WP_284522452.1	hypothetical protein	-
G5S56_RS07890 (1642683)	1642683..1643305	+	623	Protein_1536	IS3-like element IS911 family transposase	-
G5S56_RS07895 (1643300)	1643300..1643975	+	676	Protein_1537	IclR family transcriptional regulator	-
G5S56_RS07900 (1643990)	1643990..1645195	+	1206	WP_000174592.1	L-rhamnonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1633691..1664674	30983
	-	flank	IS/Tn	-	-	1642683..1642985	302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.07 Da
• Isoelectric Point: 10.3195

>T151111 WP_000513533.1 NZ_CP049278:1639834-1640082 [Shigella boydii]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T151111 NZ_CP065338:569403-569621 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13051.73 Da
• Isoelectric Point: 4.5590

>AT151111 WP_001016018.1 NZ_CP049278:1640079-1640423 [Shigella boydii]
MNHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Nucleotide

• Length: 345 bp

>AT151111 NZ_CP065338:569002-569376 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGATGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A398TMA1