Detailed information of TA system TA151086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3322401..3322990
• Replicon: chromosome
• Accession: NZ_CP049250
• Organism: Rhizobium rhizoryzae strain DSM 29514

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: G6N80_RS21950
• Protein ID: WP_165136828.1
• Coordinates: 3322401..3322733 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: G6N80_RS21955
• Protein ID: WP_165136831.1
• Coordinates: 3322733..3322990 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N80_RS21930	3317637..3318470	-	834	WP_165136819.1	ABC transporter permease subunit	-
G6N80_RS21935	3318467..3319402	-	936	WP_165136822.1	ABC transporter permease	-
G6N80_RS21940	3319399..3320919	-	1521	WP_165136825.1	ABC transporter substrate-binding protein	-
G6N80_RS21945	3321022..3322308	-	1287	WP_062552928.1	MFS transporter	-
G6N80_RS21950	3322401..3322733	-	333	WP_165136828.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N80_RS21955	3322733..3322990	-	258	WP_165136831.1	antitoxin	Antitoxin
G6N80_RS21960	3323102..3323452	+	351	WP_062552929.1	restriction endonuclease	-
G6N80_RS21965	3323581..3323883	+	303	WP_165136834.1	alkylphosphonate utilization protein	-
G6N80_RS21970	3323891..3324895	-	1005	WP_165136836.1	hypothetical protein	-
G6N80_RS21975	3324892..3325143	-	252	WP_162249528.1	acyl carrier protein	-
G6N80_RS21980	3325154..3326737	-	1584	WP_165136839.1	AMP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (5-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11691.52 Da
• Isoelectric Point: 11.4592

>T151086 WP_165136828.1 NZ_CP049250:c3322733-3322401 [Rhizobium rhizoryzae]
MNRGDIFIVSLDPTSGHEQQGTRPVLIVSPAAFNRLTRTPIVLPITSGGNFARVAGFTVSLMGVGTRTTGVIRCDQPRAL
DIGARHGRRVEAVPDAIMDEVLARLGTLIG

Nucleotide

• Length: 333 bp

>T151086 NZ_CP065228:4257678-4257929 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9109.48 Da
• Isoelectric Point: 4.0606

>AT151086 WP_165136831.1 NZ_CP049250:c3322990-3322733 [Rhizobium rhizoryzae]
MYTTSLRKVGGSIMLAVPPALLDTLHLTSGAKVGLLIEDGRLIVEPAAKPNYTLEELLAQCDPEAAPPAEDREWLDAEPV
GNELL

Nucleotide

• Length: 258 bp

>AT151086 NZ_CP065228:4257373-4257651 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA