Detailed information of TA system TA151084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 9009..9583
• Replicon: plasmid unnamed1
• Accession: NZ_CP049247
• Organism: Rhizobium rhizoryzae strain DSM 29514

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: G6N80_RS00055
• Protein ID: WP_165130327.1
• Coordinates: 9009..9263 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: G6N80_RS00060
• Protein ID: WP_165130328.1
• Coordinates: 9254..9583 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N80_RS00020	4632..4871	+	240	WP_165130320.1	hypothetical protein	-
G6N80_RS00025	5064..5747	+	684	WP_165130321.1	ParA family protein	-
G6N80_RS00030	5744..6094	+	351	WP_165130322.1	hypothetical protein	-
G6N80_RS00035	6307..6507	+	201	WP_165130323.1	hypothetical protein	-
G6N80_RS00040	6862..7572	+	711	WP_165130324.1	SOS response-associated peptidase	-
G6N80_RS00045	7584..7820	+	237	WP_165130325.1	hypothetical protein	-
G6N80_RS00050	7906..8742	-	837	WP_165130326.1	DNA adenine methylase	-
G6N80_RS00055	9009..9263	+	255	WP_165130327.1	BrnT family toxin	Toxin
G6N80_RS00060	9254..9583	+	330	WP_165130328.1	BrnA antitoxin family protein	Antitoxin
G6N80_RS00065	9627..9953	-	327	WP_165130329.1	hypothetical protein	-
G6N80_RS00070	10237..10656	-	420	WP_165130330.1	hypothetical protein	-
G6N80_RS00075	10656..11240	-	585	WP_165130331.1	lysozyme	-
G6N80_RS00080	11227..11442	-	216	WP_165130332.1	hypothetical protein	-
G6N80_RS00085	11545..12492	+	948	WP_165130333.1	DUF4214 domain-containing protein	-
G6N80_RS00090	12600..13634	-	1035	WP_165130334.1	late control D family protein	-
G6N80_RS00095	13637..13861	-	225	WP_165130335.1	tail protein X	-
G6N80_RS00100	13861..14304	-	444	WP_165130336.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54249	54249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-107)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9440.95 Da
• Isoelectric Point: 8.5905

>T151084 WP_165130327.1 NZ_CP049247:9009-9263 [Rhizobium rhizoryzae]
MKITYDENKRLVNIEKHGLDFADLTIDFFADALIVGAKQGRYMAIGEFQGIVVIAVVFRPLGSEGLSVISMRPASTTERK
HRWT

Nucleotide

• Length: 255 bp

>T151084 NZ_CP065228:2623767-2623901 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12138.76 Da
• Isoelectric Point: 5.3479

>AT151084 WP_165130328.1 NZ_CP049247:9254-9583 [Rhizobium rhizoryzae]
MDVKFSSKRPLTPEEEAEIQRLIESDPDAPEATDEALANARPFREVFPDLAASIDRERASRGRGRPPVEAPKVAVTIRLE
PEVIERFKAQGGKEWRGKMTDALRKAAGL

Nucleotide

• Length: 330 bp

>AT151084 NZ_CP065228:2623551-2623703 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA