Detailed information of TA system TA151074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 76414..77000
• Replicon: chromosome
• Accession: NZ_CP049241
• Organism: Rhizobium pseudoryzae strain DSM 19479

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: G6N78_RS00445
• Protein ID: WP_165221076.1
• Coordinates: 76414..76704 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: G6N78_RS00450
• Protein ID: WP_165214407.1
• Coordinates: 76701..77000 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N78_RS00420	72380..72775	-	396	WP_165214394.1	DUF930 domain-containing protein	-
G6N78_RS00425	72909..73181	+	273	WP_165214403.1	hypothetical protein	-
G6N78_RS00430	73389..75494	+	2106	WP_165214405.1	S9 family peptidase	-
G6N78_RS00435	75600..76046	-	447	WP_165214231.1	hypothetical protein	-
G6N78_RS25685	76181..76393	-	213	WP_206531587.1	hypothetical protein	-
G6N78_RS00445	76414..76704	+	291	WP_165221076.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6N78_RS00450	76701..77000	+	300	WP_165214407.1	putative addiction module antidote protein	Antitoxin
G6N78_RS00455	77130..78554	-	1425	WP_206531588.1	PepSY domain-containing protein	-
G6N78_RS00460	78719..79132	-	414	WP_165214409.1	hypothetical protein	-
G6N78_RS00465	79289..80548	+	1260	WP_165214411.1	DUF2254 domain-containing protein	-
G6N78_RS00470	80755..81009	+	255	WP_165214413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-89)

Antitoxin

• Uncharacterized protein HI1420 (15-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10989.84 Da
• Isoelectric Point: 10.6020

>T151074 WP_165221076.1 NZ_CP049241:76414-76704 [Rhizobium pseudoryzae]
MIEVRQHPDFVRWLRSLRDRAAQVRIAMRLVRLEAGLMGDAKFFDGIGELRIDYGPGYRVYFVKRGTRLIILLCGGDKSS
QARDIAQAKRLAEDVP

Nucleotide

• Length: 291 bp

>T151074 NZ_CP065209:c1411711-1411220 [Bifidobacterium longum subsp. longum]
ATGAGGCAGTCAAAGCAGGTGTCGATCTTGTGCGACACCAATATCCTTATTTATTACATGCTCTGTACGCCTCGGTTCAT
CAAGGATGTCATGAAACTGTTCGAGCGATGCGTCGAGCGGGAGGTAAAGCTGTCCTGTGCGTCACTGTCGCTGAAGGACG
TGTTCTACATCACCCCGGAACGATTCAAAGAACGATTCATGAATGACGAATCCATGGTCGTTGCGACCACCGCCAAGCTG
ATATTGCCGGAGGTGGCATGGTCGGGAATCAGCAAACTCAAGGAGCTCATGACAATCGTGCCGATTGGCGCGGAGGAATG
TGAATTGGCGCAGTCGTTGCGGCACGCGCATAACGATTTCGAGGATGACCTTATTATCGCCGCCGCACAACAATCCGGGG
CCACCTATGTGGTGACGTATGACAAAAAACTAGCCAAGCATTTTCCCGAATATTGCAAAACGCCGGCTGAAATCGTGGCG
GCGCTGGCATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10360.88 Da
• Isoelectric Point: 4.4488

>AT151074 WP_165214407.1 NZ_CP049241:76701-77000 [Rhizobium pseudoryzae]
MTVEPIPFEASEFFETVDSQALLLSDALETGDCAYIAHAFGVIAKARGMTGIAREAGVTREALYKALSEKGDPKLSTLLG
VAKALGLKFTVVPASDAAE

Nucleotide

• Length: 300 bp

>AT151074 NZ_CP065209:c1412100-1411708 [Bifidobacterium longum subsp. longum]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTGTACATCGCCGACCGCAAGGAAGTGCCGGCCTTGGAAACGACCGCCG
AAGAGCAAGCCCGCGAGGAGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGTGCCGGATACGTACACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGATGAGATAGCTGA
CGAACGGCTGAAGGAATACAGGCATCAGTCGGACTATGCGCGTTATTTGGAGAAGGAGCGGAGTCTGTCATGA