Detailed information of TA system TA151072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 6094..6743
• Replicon: chromosome
• Accession: NZ_CP049241
• Organism: Rhizobium pseudoryzae strain DSM 19479

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N78_RS00055
• Protein ID: WP_165214266.1
• Coordinates: 6357..6743 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N78_RS00050
• Protein ID: WP_165214264.1
• Coordinates: 6094..6357 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N78_RS00015	2392..2847	-	456	WP_165214250.1	C40 family peptidase	-
G6N78_RS00020	2844..3716	-	873	WP_165214252.1	DUF2163 domain-containing protein	-
G6N78_RS00025	3713..4348	-	636	WP_165214254.1	DUF2460 domain-containing protein	-
G6N78_RS00030	4361..4921	-	561	WP_165214256.1	phage tail tape measure protein	-
G6N78_RS00035	4971..5204	-	234	WP_165214258.1	phage tail assembly chaperone	-
G6N78_RS00040	5201..5563	-	363	WP_165214260.1	gene transfer agent family protein	-
G6N78_RS00045	5563..5970	-	408	WP_165214262.1	phage major tail protein, TP901-1 family	-
G6N78_RS00050	6094..6357	+	264	WP_165214264.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
G6N78_RS00055	6357..6743	+	387	WP_165214266.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N78_RS00060	6958..8094	+	1137	WP_165221064.1	MFS transporter	-
G6N78_RS00065	8075..8473	-	399	WP_165214268.1	DUF3168 domain-containing protein	-
G6N78_RS00070	8470..8808	-	339	WP_165214270.1	phage head closure protein	-
G6N78_RS00075	8809..9378	-	570	WP_165214272.1	phage head-tail connector protein	-
G6N78_RS00080	9462..10538	-	1077	WP_165214274.1	ISAs1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2392..19763	17371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (2-82)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13773.71 Da
• Isoelectric Point: 4.8432

>T151072 WP_165214266.1 NZ_CP049241:6357-6743 [Rhizobium pseudoryzae]
MVIDSSAITAILANEADAESLERRLLADPVRLISAANFLELAIVIEAKFGDAGAKELNTWFELAAVNIVPVDAALADVAR
QAWRRFGKGRHAAALNFGDCFSYAMAKAHNQPLLFKGNDFSQTDIPAA

Nucleotide

• Length: 387 bp

>T151072 NZ_CP065202:c4934250-4933972 [Pseudomonas fragi]
ATGATCGTAAGCTTTAAATGCAACAGAACGGAACGTCTGTATCGAAGCGGCAAGACGCGCTTATGGTCAGATATTCTCAA
TGTCATTGAACGAAAATTAACCATGCTGGACGCTGCCAGCGTGCTGACAGATTTGGCGTCCCCCCCTGGCAACCGCCTCG
AGGCACTCGGCGGAGCTCGCAAGGGCCAATACAGCATCCGTATCAACGCACAATGGCGAATCTGCTTTATCTGGGGCGCC
AAAGGCCCTGAAGCAGTAGAAATCGTCGATTACCATTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9393.59 Da
• Isoelectric Point: 5.1745

>AT151072 WP_165214264.1 NZ_CP049241:6094-6357 [Rhizobium pseudoryzae]
VALSIRDSETERLARALAAATGESVAIATRKALEERLSRLPETPPAPAERLAERLAASRRRFAALPVLDPRTPDEILGYD
EQSGTPS

Nucleotide

• Length: 264 bp

>AT151072 NZ_CP065202:c4933964-4933659 [Pseudomonas fragi]
ATGATCAAGAACGGCATGCGCCCGGTTCACCCGGGTGAAGTTCTCAAGGAAGAATACCTTGAGCCCCTGAGCCTCTCGTC
CGCTGCCTTGGCCAGGGCGCTCGGGGTGTCTACACCGACGGTCAACGATATCGTGCTGCAACGCCGTGGCGTCAGTGCCG
ATATGGCGTTGCGACTGTCGGTCTGCCTGGGGACAACCCCCGAGTTCTGGCTGAACTTGCAAACCACTTACGACCTGCGA
AAAGCCGAAATCGACCGCGGCGCCGCGATTCGCGAACAGGTGCAGCCGATTCACCACTGCGCCTGA