Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 100079..100749 Replicon chromosome
Accession NZ_CP049207
Organism Agrobacterium rubi strain W2/73

Toxin (Protein)


Gene name vapC Uniprot ID A0A1B9TYX8
Locus tag G6M88_RS14825 Protein ID WP_065698538.1
Coordinates 100079..100498 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B9TYY3
Locus tag G6M88_RS14830 Protein ID WP_065698539.1
Coordinates 100495..100749 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M88_RS14810 (G6M88_14830) 95623..98169 + 2547 WP_234791123.1 excinuclease ABC subunit UvrA -
G6M88_RS14815 (G6M88_14835) 98275..98574 - 300 WP_065698632.1 antibiotic biosynthesis monooxygenase -
G6M88_RS14820 (G6M88_14840) 98801..99988 + 1188 WP_065698537.1 integrase core domain-containing protein -
G6M88_RS14825 (G6M88_14845) 100079..100498 - 420 WP_065698538.1 type II toxin-antitoxin system VapC family toxin Toxin
G6M88_RS14830 (G6M88_14850) 100495..100749 - 255 WP_065698539.1 plasmid stabilization protein Antitoxin
G6M88_RS14835 (G6M88_14855) 101162..102229 + 1068 WP_234791124.1 Fic family protein -
G6M88_RS14840 102392..102818 + 427 Protein_106 integrase -
G6M88_RS14845 (G6M88_14865) 103187..103486 - 300 WP_065698542.1 DUF982 domain-containing protein -
G6M88_RS14850 (G6M88_14870) 103563..104036 - 474 WP_045232133.1 Hsp20 family protein -
G6M88_RS14855 (G6M88_14875) 104366..105442 - 1077 WP_065698543.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14866.09 Da        Isoelectric Point: 5.6993

>T151057 WP_065698538.1 NZ_CP049207:c100498-100079 [Agrobacterium rubi]
MILLDTNVISEPWKPAPGIAVLTWLDAQAIETLFISAISVAELRFGIASMPSGKRQTILHDRLEGEVLPIFDGRILPFNK
GTSQFYADLMASGRTSGKAIGKADGYIAATAAENHLAVATRDTSPFEAAGLRVINPWAA

Download         Length: 420 bp

>T151057 NZ_CP065194:c2820976-2820515 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9231.41 Da        Isoelectric Point: 7.2047

>AT151057 WP_065698539.1 NZ_CP049207:c100749-100495 [Agrobacterium rubi]
MAAVTIRNLSDETHRALRARAAYHGRSTEAEIRDILEAAVRPSGRVKLGSLLAAIGRDAEMSNSDIEALQQQRDRTPAVP
MTFE

Download         Length: 255 bp

>AT151057 NZ_CP065194:c2821303-2820989 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAGCGTTATGAAAGCGGAAATATCAAAAATTTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCATATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCACCTT
GAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B9TYX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1B9TYY3

References