Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 2757962..2758506 Replicon chromosome
Accession NZ_CP049206
Organism Agrobacterium rubi strain W2/73

Toxin (Protein)


Gene name mazF Uniprot ID A0A1B9U0A2
Locus tag G6M88_RS13220 Protein ID WP_065698338.1
Coordinates 2758183..2758506 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1B9U082
Locus tag G6M88_RS13215 Protein ID WP_065698339.1
Coordinates 2757962..2758186 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M88_RS13210 (G6M88_13230) 2757163..2757741 - 579 WP_234791118.1 hypothetical protein -
G6M88_RS13215 (G6M88_13235) 2757962..2758186 + 225 WP_065698339.1 antitoxin MazE family protein Antitoxin
G6M88_RS13220 (G6M88_13240) 2758183..2758506 + 324 WP_065698338.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
G6M88_RS13225 (G6M88_13245) 2758540..2758833 - 294 WP_065698337.1 transcriptional regulator -
G6M88_RS13230 (G6M88_13250) 2759356..2759727 + 372 WP_042618537.1 30S ribosomal protein S12 -
G6M88_RS13235 (G6M88_13255) 2759805..2760275 + 471 WP_045232852.1 30S ribosomal protein S7 -
G6M88_RS13240 (G6M88_13260) 2760303..2762402 + 2100 WP_045232851.1 elongation factor G -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-101)

Antitoxin

Antitoxin MazE-like

(6-71)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11558.50 Da        Isoelectric Point: 9.9929

>T151056 WP_065698338.1 NZ_CP049206:2758183-2758506 [Agrobacterium rubi]
VRRGDLVTVSVSGDFGKPRPALVIQSDYFSDTATVTVLLLSGTLVDAPFIRLTVQPSSRNGLTKPSQVMIDKSMTIVRDK
IGPVFGRLEDDMMLSITRSMAVFVGVA

Download         Length: 324 bp

>T151056 NZ_CP065194:c2145844-2145737 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8194.08 Da        Isoelectric Point: 4.5580

>AT151056 WP_065698339.1 NZ_CP049206:2757962-2758186 [Agrobacterium rubi]
MATSVNHRVQKRRDALRASGLRPVQIWVPDTRRAGFAEECLRQSKAVAVSDAFDTEFDVFADAALADLDDEASE

Download         Length: 225 bp

>AT151056 NZ_CP065194:2145660-2145720 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B9U0A2

Antitoxin

Source ID Structure
AlphaFold DB A0A1B9U082

References