Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2664282..2664913 Replicon chromosome
Accession NZ_CP049206
Organism Agrobacterium rubi strain W2/73

Toxin (Protein)


Gene name vapC Uniprot ID A0A1B9U0G2
Locus tag G6M88_RS12815 Protein ID WP_065698385.1
Coordinates 2664515..2664913 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B9U0H7
Locus tag G6M88_RS12810 Protein ID WP_065698386.1
Coordinates 2664282..2664515 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M88_RS12785 (G6M88_12805) 2659364..2659600 + 237 WP_006727438.1 acyl carrier protein -
G6M88_RS12790 (G6M88_12810) 2659844..2661112 + 1269 WP_045231833.1 beta-ketoacyl-ACP synthase II -
G6M88_RS12795 (G6M88_12815) 2661361..2662584 + 1224 WP_065698388.1 endolytic transglycosylase MltG -
G6M88_RS12800 (G6M88_12820) 2662641..2663525 + 885 WP_065698387.1 YicC/YloC family endoribonuclease -
G6M88_RS12805 (G6M88_12825) 2663531..2664202 + 672 WP_045231836.1 guanylate kinase -
G6M88_RS12810 (G6M88_12830) 2664282..2664515 + 234 WP_065698386.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G6M88_RS12815 (G6M88_12835) 2664515..2664913 + 399 WP_065698385.1 tRNA(fMet)-specific endonuclease VapC Toxin
G6M88_RS12820 (G6M88_12840) 2664924..2665766 - 843 WP_065698384.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
G6M88_RS12825 (G6M88_12845) 2665766..2666785 - 1020 WP_065698464.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
G6M88_RS12830 (G6M88_12850) 2666803..2667747 - 945 WP_065698383.1 peptidylprolyl isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(8-56)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14809.14 Da        Isoelectric Point: 6.6961

>T151054 WP_065698385.1 NZ_CP049206:2664515-2664913 [Agrobacterium rubi]
MLTYMLDTNICIYVMKTYPPELRDKFNAMAEQLCISSITLGELCYGAEKSARRNENLVAIDNFVSRLDVLPFADKAAAHY
GQIRAELARAGTPCGVHDMQIGGHARSEGLIVVTNNMREFIRMPGLRVENWV

Download         Length: 399 bp

>T151054 NZ_CP065194:c1790180-1789971 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8812.03 Da        Isoelectric Point: 8.8756

>AT151054 WP_065698386.1 NZ_CP049206:2664282-2664515 [Agrobacterium rubi]
MITSTVFLSNRSQAVRLPKAVAFPEDVHQVDILKIGRTRIIVPHGKRWDDLFASGPRASKDFLSDREQPSAEEREFF

Download         Length: 234 bp

>AT151054 NZ_CP065194:c1790485-1790192 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B9U0G2

Antitoxin

Source ID Structure
AlphaFold DB A0A1B9U0H7

References