Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 2483705..2484330 Replicon chromosome
Accession NZ_CP049206
Organism Agrobacterium rubi strain W2/73

Toxin (Protein)


Gene name mazF Uniprot ID A0A1B9TWC0
Locus tag G6M88_RS11865 Protein ID WP_065698895.1
Coordinates 2483971..2484330 (+) Length 120 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1B9TWA5
Locus tag G6M88_RS11860 Protein ID WP_065698896.1
Coordinates 2483705..2483971 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M88_RS11830 (G6M88_11850) 2478885..2479700 + 816 WP_065698902.1 penicillin-binding transpeptidase domain-containing protein -
G6M88_RS11835 (G6M88_11855) 2479704..2479988 - 285 WP_065698901.1 type II toxin-antitoxin system YafQ family toxin -
G6M88_RS11840 (G6M88_11860) 2480036..2480311 - 276 WP_065698900.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
G6M88_RS11845 (G6M88_11865) 2480455..2482956 + 2502 WP_065698899.1 heavy metal translocating P-type ATPase -
G6M88_RS11850 (G6M88_11870) 2482953..2483363 + 411 WP_065698898.1 Cu(I)-responsive transcriptional regulator -
G6M88_RS11855 (G6M88_11875) 2483390..2483590 + 201 WP_065698897.1 heavy-metal-associated domain-containing protein -
G6M88_RS11860 (G6M88_11880) 2483705..2483971 + 267 WP_065698896.1 PbsX family transcriptional regulator Antitoxin
G6M88_RS11865 (G6M88_11885) 2483971..2484330 + 360 WP_065698895.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
G6M88_RS11870 (G6M88_11890) 2484339..2484527 + 189 WP_065698894.1 hypothetical protein -
G6M88_RS11875 (G6M88_11895) 2484543..2484791 - 249 WP_065698893.1 hypothetical protein -
G6M88_RS11880 (G6M88_11900) 2484931..2486346 + 1416 WP_162273323.1 MFS transporter -
G6M88_RS11885 (G6M88_11905) 2486341..2486511 - 171 WP_164470675.1 hypothetical protein -
G6M88_RS11890 (G6M88_11910) 2486578..2488293 - 1716 WP_065698892.1 GH32 C-terminal domain-containing protein -
G6M88_RS11895 (G6M88_11915) 2488354..2488569 - 216 WP_141680619.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 12866.06 Da        Isoelectric Point: 8.2958

>T151053 WP_065698895.1 NZ_CP049206:2483971-2484330 [Agrobacterium rubi]
MVRSNVPKRGDVFLIDLNPVIGNEMNDEHRCVVITPKEINAVGLCLVVPITTGGMFTRKAGLAVNISGHRTTGVALCNQV
RSLDIAGRIAQKRAKFLETLDDATIQEIVARVVSMIDPA

Download         Length: 360 bp

>T151053 NZ_CP065194:c1770596-1770234 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9384.86 Da        Isoelectric Point: 4.7389

>AT151053 WP_065698896.1 NZ_CP049206:2483705-2483971 [Agrobacterium rubi]
MTVTTKIRRQGGAAVMTIPPALLKLMGTEIGSEMTLEIDNGNLVATPVTTQKKRYSLAELLEGADEMAELNVEASAWNAA
PRVGKEAL

Download         Length: 267 bp

>AT151053 NZ_CP065194:c1770763-1770593 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B9TWC0

Antitoxin

Source ID Structure
AlphaFold DB A0A1B9TWA5

References