151049

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 2191022..2191508 Replicon chromosome
Accession NZ_CP049206
Organism Agrobacterium rubi strain W2/73

Toxin (Protein)


Gene name pasB Uniprot ID A0A1B9THM3
Locus tag G6M88_RS10385 Protein ID WP_065699736.1
Coordinates 2191022..2191291 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID A0A1B9THJ9
Locus tag G6M88_RS10390 Protein ID WP_065699735.1
Coordinates 2191275..2191508 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M88_RS10355 (G6M88_10375) 2186355..2187677 + 1323 WP_065699741.1 glycolate oxidase subunit GlcF -
G6M88_RS10360 (G6M88_10380) 2187844..2188566 - 723 WP_065699740.1 L,D-transpeptidase -
G6M88_RS10365 (G6M88_10385) 2188756..2189385 + 630 WP_065699739.1 DNA-3-methyladenine glycosylase I -
G6M88_RS10370 (G6M88_10390) 2189382..2189825 + 444 WP_065699738.1 hypothetical protein -
G6M88_RS10375 (G6M88_10395) 2189833..2190207 - 375 WP_065700120.1 DUF305 domain-containing protein -
G6M88_RS10380 (G6M88_10400) 2190254..2191018 - 765 WP_065699737.1 hypothetical protein -
G6M88_RS10385 (G6M88_10405) 2191022..2191291 - 270 WP_065699736.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G6M88_RS10390 (G6M88_10410) 2191275..2191508 - 234 WP_065699735.1 DUF6290 family protein Antitoxin
G6M88_RS10395 (G6M88_10415) 2191665..2193284 + 1620 WP_065699734.1 histidine--tRNA ligase -
G6M88_RS10400 (G6M88_10420) 2193289..2193660 + 372 WP_065699733.1 VOC family protein -
G6M88_RS10405 (G6M88_10425) 2193677..2194798 + 1122 WP_065699732.1 ATP phosphoribosyltransferase regulatory subunit -
G6M88_RS10410 (G6M88_10430) 2194795..2195490 + 696 WP_065699731.1 ATP phosphoribosyltransferase -
G6M88_RS10415 (G6M88_10435) 2195563..2196009 - 447 WP_065699730.1 DoxX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-88)

Antitoxin

Protein of unknown function DUF6290

(6-72)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10562.21 Da        Isoelectric Point: 9.8727

>T151049 WP_065699736.1 NZ_CP049206:c2191291-2191022 [Agrobacterium rubi]
MAWRIEFQEKPLKQLEKLGKTNASRIISFLTDRIALDEDPRRSGIALRGSELGNYWRYRVGDYRILCDIQDNKLVVLVIE
IGHRREVYR

Download         Length: 270 bp

>T151049 NZ_CP065178:4316057-4316308 [Pectobacterium quasiaquaticum]
GTGCATGATATAAAAAAGATTGAATACAAAACTTTATCCGAGCAGCAGCTTGAAAACATACTGACCGGATATCTGTCCGA
TGCCCCGCTGAGTATGATTGATACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCGCTGCTTT
ATCTGGATGATCGCTGGTGCCTGCCTCTCAATGCCACACCAACTACCCACATCATCAAGCTACCGATTGGTAAAATCGAG
AGTCACTCTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 9123.15 Da        Isoelectric Point: 4.7359

>AT151049 WP_065699735.1 NZ_CP049206:c2191508-2191275 [Agrobacterium rubi]
MSKQTAIRLPDDTYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRGRLENNERTYTLEEVKQRLGLEN

Download         Length: 234 bp

>AT151049 NZ_CP065178:4315750-4316019 [Pectobacterium quasiaquaticum]
ATGATGAAGATCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACCCAGAAGCTTTCACAAGGGAA
AGTGGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGCTGGAAACCT
TCTTTAAAATCCTGTCGGCATTGAATTTAGAACTTACTGTTACGCCCAGGAATGTGGACACCACCAATAACGAAGCCTCT
GTTGCATCATCCTGGAAGGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B9THM3

Antitoxin

Source ID Structure
AlphaFold DB A0A1B9THJ9

References