Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 972589..973197 Replicon chromosome
Accession NZ_CP049206
Organism Agrobacterium rubi strain W2/73

Toxin (Protein)


Gene name PumA Uniprot ID A0A1B9U1R1
Locus tag G6M88_RS04725 Protein ID WP_065697660.1
Coordinates 972589..972885 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1B9U1N8
Locus tag G6M88_RS04730 Protein ID WP_065697659.1
Coordinates 972889..973197 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M88_RS04695 (G6M88_04695) 967678..968460 - 783 WP_065697666.1 heme ABC transporter ATP-binding protein -
G6M88_RS04700 (G6M88_04700) 968457..969548 - 1092 WP_065697665.1 iron ABC transporter permease -
G6M88_RS04705 (G6M88_04705) 969545..970432 - 888 WP_065697664.1 ABC transporter substrate-binding protein -
G6M88_RS04710 (G6M88_04710) 970610..970963 + 354 WP_065697663.1 antibiotic biosynthesis monooxygenase -
G6M88_RS04715 (G6M88_04715) 970960..971466 + 507 WP_065697662.1 heme utilization cystosolic carrier protein HutX -
G6M88_RS04720 (G6M88_04720) 971653..972537 + 885 WP_065697661.1 formyltetrahydrofolate deformylase -
G6M88_RS04725 (G6M88_04725) 972589..972885 + 297 WP_065697660.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G6M88_RS04730 (G6M88_04730) 972889..973197 + 309 WP_065697659.1 putative addiction module antidote protein Antitoxin
G6M88_RS04735 (G6M88_04735) 973292..973690 + 399 WP_065697658.1 hypothetical protein -
G6M88_RS04740 (G6M88_04740) 973730..975106 - 1377 WP_065697657.1 sensor histidine kinase -
G6M88_RS04745 (G6M88_04745) 975103..975783 - 681 WP_065698219.1 response regulator transcription factor -
G6M88_RS04750 (G6M88_04750) 975868..976935 + 1068 WP_065698218.1 ABC transporter substrate-binding protein -
G6M88_RS04755 (G6M88_04755) 977180..978124 + 945 WP_065697656.1 tripartite tricarboxylate transporter substrate binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11026.73 Da        Isoelectric Point: 10.0286

>T151047 WP_065697660.1 NZ_CP049206:972589-972885 [Agrobacterium rubi]
MFTIRETEEFADWLAGLRDVQARARIARRLYRLADGNPGDVKPVGESVSELRIDYGPGYRVYFIQDGKVVIVLLCGGDKS
SQRRDIAKAKKLAKALKE

Download         Length: 297 bp

>T151047 NZ_CP065178:c3887312-3886827 [Pectobacterium quasiaquaticum]
ATGGTTGATAAGCGAGTAATAAATGGGTGGCAGCTATTTACGCATCCTTGTTTTGATGCGCAGTTTGAGTCTCTTATTGT
TGAAGTTGAGCAACTGCGCAAGAAATATCCAGAATCTTACCAGAAAAAGCAGGCAACTAAGCTATTGGCTGCAATAGTCA
AAGTCATCCGTACTGAGATTTGTGGCGATCCGTCCCAAACGAAGTTTAGACAAGGCGATACGCTAGGCGGAGGTAACAAA
CATTGGTTCAGGGCAAAATTTCTGGGTCAGTATCGGTTATTTTTTCGCTATAGCGAGAGACATAAAACTATCATCCTTGC
CTGGGTCAATGATTCAGACACTAAACGAGCATATGGCGATCAGCATGATGCTTATAAAGTTTTTAGCGGCATGCTAGCAA
GCGGGAATCCGCCGGGAGATTGGGAGCAACTGCTCGCTGAATCCCATAGTGAGGCCATGCTTACGCAAATGCTCGTGCTG
CCGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 10740.05 Da        Isoelectric Point: 4.5994

>AT151047 WP_065697659.1 NZ_CP049206:972889-973197 [Agrobacterium rubi]
VPLATREYDATRYLDSEEAIAAYIADATASGDANELAHALGVVARAKGMSDLSRQTGLSRQTLYKALSGESNPELGTIAK
VADALGYRLSLVAKSPEIIESL

Download         Length: 309 bp

>AT151047 NZ_CP065178:c3887662-3887318 [Pectobacterium quasiaquaticum]
ATGTTTGATTTAAAATCAGCGGATGTGTTGTTGCGCGCCGATAGTCGCTTAACGGCGCGTAGTCAGACAACGATTCCATC
GTCTGTGCGTGATGCAATGAACCTGCAACCTGGGGAGTACATTCATTATAGCGTGCTACCGGGGGGGCAAGTGCTGATCA
GTCGGCGTGAAGAGGTGATAGATGATGACCCCGTTATTCATCACTTTTTGCATTTTTTGGCTGAAGATATGCAACGTCAT
CCAGAAAATATTAAGCCGTTTGATCAATCGCTTATGTCACGTGCGAGTGTGTTAACCGCAGGGATGGATATTGATCTGGA
TGCACCGCTTACTGACGACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B9U1R1

Antitoxin

Source ID Structure
AlphaFold DB A0A1B9U1N8

References