Detailed information of TA system TA151045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-higA/BrnT(toxin)
• Location: 386039..386563
• Replicon: chromosome
• Accession: NZ_CP049206
• Organism: Agrobacterium rubi strain W2/73

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: G6M88_RS01830
• Protein ID: WP_234791112.1
• Coordinates: 386039..386260 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1B9U3T7
• Locus tag: G6M88_RS01835
• Protein ID: WP_065698275.1
• Coordinates: 386330..386563 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6M88_RS01820 (G6M88_01820)	384836..385201	+	366	WP_065698078.1	helix-turn-helix transcriptional regulator	-
G6M88_RS01825 (G6M88_01825)	385168..385752	+	585	WP_083212526.1	ImmA/IrrE family metallo-endopeptidase	-
G6M88_RS01830 (G6M88_01830)	386039..386260	+	222	WP_234791112.1	BrnT family toxin	Toxin
G6M88_RS01835 (G6M88_01835)	386330..386563	+	234	WP_065698275.1	transcriptional regulator	Antitoxin
G6M88_RS01840 (G6M88_01840)	386573..386845	-	273	WP_065698075.1	hypothetical protein	-
G6M88_RS01845 (G6M88_01845)	387151..387732	-	582	WP_235695185.1	DUF1349 domain-containing protein	-
G6M88_RS01850 (G6M88_01850)	388171..389370	-	1200	WP_065698073.1	FAD-dependent monooxygenase	-
G6M88_RS01855 (G6M88_01855)	389396..389893	-	498	WP_065698072.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	385213..407657	22444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (1-64)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8385.34 Da
• Isoelectric Point: 7.2031

>T151045 WP_234791112.1 NZ_CP049206:386039-386260 [Agrobacterium rubi]
ISFANASTVFDDPFALDMEDRSINYGEVRRRIIGIGHDRFLTVIHTERGDTIRLISARKATRSERREYSDVNG

Nucleotide

• Length: 222 bp

>T151045 NZ_CP065178:c3444162-3443746 [Pectobacterium quasiaquaticum]
GTGAGTCGCTTGTTCATGCTGGATACCAATATTTGTTCCTTCATTATGCGGGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAATGCACGCTGCGGCGTGATCGTATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGTA
AAAAAGCCTCGCCACGCCATATGCAACTGGTCGATGCTTTTTGCCGTCGGCTCGATGCGGTATTACCTTGGGATAGCGCC
GCAGTAGATGCCACCACGAAAATCAAAATCGAGCTAGCCGCCGCTGGCACGCCTATTGGTCCAAACGATACGGCCATCGC
GGGCCATGCCATCAGCGCTAAGGCCATATTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8699.94 Da
• Isoelectric Point: 10.6759

>AT151045 WP_065698275.1 NZ_CP049206:386330-386563 [Agrobacterium rubi]
MTQEQLRSAPRIPQVNVIRRALRLTQEEFSARYQIPLGTLRDWEQSRSEPDQHARAYLKVIAVDPAGTAAALKKSAV

Nucleotide

• Length: 234 bp

>AT151045 NZ_CP065178:c3444386-3444159 [Pectobacterium quasiaquaticum]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTGCGTCCTGTTAAACCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCCGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCAAACTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B9U3T7