Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4369021..4369518 Replicon chromosome
Accession NZ_CP049204
Organism Escherichia coli strain PapM-32-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G6Z93_RS20755 Protein ID WP_001173343.1
Coordinates 4369021..4369194 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G6Z93_RS20760 Protein ID WP_000398619.1
Coordinates 4369222..4369518 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Z93_RS20745 4365691..4367148 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
G6Z93_RS20750 4367385..4368902 + 1518 WP_001295074.1 lysine--tRNA ligase -
G6Z93_RS20755 4369021..4369194 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G6Z93_RS20760 4369222..4369518 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G6Z93_RS20765 4369745..4370017 - 273 WP_000405647.1 N-acetyltransferase -
G6Z93_RS20770 4370029..4370259 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
G6Z93_RS20775 4370440..4372071 + 1632 WP_001216470.1 sensor histidine kinase -
G6Z93_RS20780 4372068..4372787 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T151039 WP_001173343.1 NZ_CP049204:c4369194-4369021 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T151039 NZ_CP065178:1566523-1566843 [Pectobacterium quasiaquaticum]
ATGCAAACATCACCTGCAATCCCGCCACGGGAGGATAACCCCTGTCCGTCACCTATAGCCGTCTGGCAGCAACTGCTTAC
CTACCTGTTAGAAAAGCACTACGGCCTGACGCTGAACGACACACCGTTCTGTGAGGAAAACGTCATTCAGGCGCATATCG
ATGCTGGCGTCACATTGGTCAATGCCGTGAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTTACCGCCGTTGATGTTCTCCGTGCCAGACGTGCTATCGGCTTGCTCAAGGCATA
A

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT151039 WP_000398619.1 NZ_CP049204:c4369518-4369222 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT151039 NZ_CP065178:1566118-1566453 [Pectobacterium quasiaquaticum]
ATGCCATCATCGGATACCCCGGAGTGGGGATTAAAATGTGCCATCACTCGGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCACTATCTGGCAGACCGGGCCAGCATTGTCGGTACGTTTAGCAAAACAGAAGCCCAAAGTCTGGAGCGTT
GTTTCCCTGAGCTAATTGAGCTGTTGGAACAGAAGCTACGCATGGGGGAACTGAATCCCCGTCAGCAGGGGGGTGTCACG
TTGTATTGTAACGAATGGACCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTATATTGTTATTTATCCTTCTTC
AGCGGCAACACAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References