Detailed information of TA system TA151035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4018310..4018860
• Replicon: chromosome
• Accession: NZ_CP049204
• Organism: Escherichia coli strain PapM-32-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: G6Z93_RS19085
• Protein ID: WP_001160975.1
• Coordinates: 4018310..4018624 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: G6Z93_RS19090
• Protein ID: WP_000125565.1
• Coordinates: 4018627..4018860 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z93_RS19060	4013985..4015271	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
G6Z93_RS19065	4015271..4016260	+	990	WP_001752008.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
G6Z93_RS19070	4016257..4017078	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
G6Z93_RS19075	4017081..4017458	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
G6Z93_RS19080	4017465..4018313	+	849	WP_021556397.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
G6Z93_RS19085	4018310..4018624	-	315	WP_001160975.1	CcdB family protein	Toxin
G6Z93_RS19090	4018627..4018860	-	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G6Z93_RS19095	4018946..4019425	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
G6Z93_RS19100	4019617..4021479	-	1863	WP_000377186.1	glutathione-regulated potassium-efflux system protein KefC	-
G6Z93_RS19105	4021472..4022002	-	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
G6Z93_RS19110	4022113..4023444	-	1332	WP_001183198.1	MFS transporter	-
G6Z93_RS19115	4023503..4023790	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T151035 WP_001160975.1 NZ_CP049204:c4018624-4018310 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T151035 NZ_CP065178:670628-671002 [Pectobacterium quasiaquaticum]
ATGCAACTGTTATCATTGGTTGTGCATGGCCTATTAGTATTGCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCATG
GCTATGGCTATGCCTAGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAAAAGAATATCAAATCCCGACAGGGGGAAA
TCGTTCTGCTTAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAAT
GGCGTATTGCTGTCATTGCAAGCGGTTAACGGTAAAGACAAACAACAGCTGTGGCTGGCATCGGACAGCATGGGCGATGA
CGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAACACTGGGCAAGATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT151035 WP_000125565.1 NZ_CP049204:c4018860-4018627 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT151035 NZ_CP065178:670342-670608 [Pectobacterium quasiaquaticum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACGGATAAGCGCACGTTTGTACGCTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCGATGTGA

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7