Detailed information of TA system TA151022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1049027..1049610
• Replicon: chromosome
• Accession: NZ_CP049204
• Organism: Escherichia coli strain PapM-32-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: G6Z93_RS04975
• Protein ID: WP_000254738.1
• Coordinates: 1049275..1049610 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: G6Z93_RS04970
• Protein ID: WP_000581937.1
• Coordinates: 1049027..1049275 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z93_RS04960	1045366..1046667	+	1302	WP_069913215.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
G6Z93_RS04965	1046715..1048949	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
G6Z93_RS04970	1049027..1049275	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
G6Z93_RS04975	1049275..1049610	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
G6Z93_RS04980	1049681..1050472	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
G6Z93_RS04985	1050700..1052337	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
G6Z93_RS04990	1052425..1053723	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T151022 WP_000254738.1 NZ_CP049204:1049275-1049610 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T151022 NZ_CP065177:c3404546-3404043 [Pectobacterium quasiaquaticum]
ATGCATCTTGGTTTAACAGGGCTATATCAGCCAAAGTGGACGGCACTAATTCAGGAAGAGTGGCAGCGAAATTTGTTGCT
AAATAATGCAGACGTAACGATGGATAAGCTCAGGAACATTGAGCATTTGATGAATAAGGCATTACCTGATGCCAATGTGA
CTGGTTTCGAACAACTTATCCCTGCTCTGGTTTTACCTGATGCGGATGATCGACATGTATTGGCCGCTGCGATACGCGCT
AATGCAGAAGTTATCGTCACGGCAAATCTGAAAGATTTTCCAGCAACGGCACTTTCCGAGTTTGCGGTGGAAGCCATGCA
CCCAGACGAGTTTATTGCCGATTTATTTGATCTCAATTCTGCACTGGTACTTAAAGCCGTTCGAACTTCACGCACAGCTT
TCAGAAAGCCTCCATTTGATGTGGACGAGTATCTGGATTGTCTCCTGCGTCAGGGGTTACCTATGACGGTAAAAGAACTG
GATGCGTATCGAGCACTGATTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT151022 WP_000581937.1 NZ_CP049204:1049027-1049275 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT151022 NZ_CP065177:c3405079-3404618 [Pectobacterium quasiaquaticum]
ATGAAAAATACTCCTCTAAATAGTTTGAGCCTTCCGGCTCCAAAAGAAATGGAGGCCGCTATTCGTGGACAAAGGCAGCT
TGCAACCTACTTGTCTACGAAGCTGGAAACACAGGAAATTTCGATTCAGGATGCACATGATATTGCCCATCGGATCGAGT
TGCCTACCTCAGCACTCACGCTTTTGATGAGCATCCTTGGCGAACTGGCGGCGGGTAATGCTGTCCAGATTGTCCCCGTG
CAGGCTGAGTTAACGACACAAGAAGCGGCAAATATTCTGAATGTTTCACGGCCACATATGGTGAAGCTTTTGGAAGAAGG
GCATCTTCCTTTCCATAAAACAGGTCGTCATCGCCGCGTGCTTTTTGCTGATTTGATGCAATACAAAGAACAGCGTGAAA
GTGAAAGTATGCAAGCCATGCAGGAACTGACAGACCGAAGTCAGGAACTCGGGTTTTACTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4