Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 892947..893601 Replicon chromosome
Accession NZ_CP049204
Organism Escherichia coli strain PapM-32-1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag G6Z93_RS04290 Protein ID WP_000244777.1
Coordinates 893194..893601 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6Z93_RS04285 Protein ID WP_000354046.1
Coordinates 892947..893213 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Z93_RS04265 889035..890468 - 1434 WP_021557183.1 6-phospho-beta-glucosidase BglA -
G6Z93_RS04270 890513..890824 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
G6Z93_RS04275 890988..891647 + 660 WP_000250274.1 hemolysin III family protein -
G6Z93_RS04280 891724..892704 - 981 WP_000886074.1 tRNA-modifying protein YgfZ -
G6Z93_RS04285 892947..893213 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6Z93_RS04290 893194..893601 + 408 WP_000244777.1 toxin CptA Toxin
G6Z93_RS04295 893641..894162 - 522 WP_001613762.1 flavodoxin FldB -
G6Z93_RS04300 894274..895170 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G6Z93_RS04305 895195..895905 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6Z93_RS04310 895911..897644 + 1734 WP_000813202.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T151021 WP_000244777.1 NZ_CP049204:893194-893601 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T151021 NZ_CP065177:3225589-3225792 [Pectobacterium quasiaquaticum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACTATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCCAATGATGAACTGGAGATGTTCTTTTCCGCAGCAGATCATCGTCTGGCAGAACTGACGATGAACAAAC
TCTACGACAAAGTCCCTACAGCAGTATGGCGATACGTTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT151021 WP_000354046.1 NZ_CP049204:892947-893213 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT151021 NZ_CP065177:3225168-3225536 [Pectobacterium quasiaquaticum]
ATGGATGAGTACACACCAAAATATTATGATATTGCCCAACTCAGATTCTTATGTGAAAATCTGTGTGACGAAAGCATAGC
AACGTTAGGTGATAGCAGTCACGGCTGGGTCAATGATCCGACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGAATTAATGATGCTGAACTGCGACGTTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACATCTGTATGCCTGCTAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References