Detailed information of TA system TA151019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 341246..342046
• Replicon: chromosome
• Accession: NZ_CP049204
• Organism: Escherichia coli strain PapM-32-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: G6Z93_RS01530
• Protein ID: WP_000342448.1
• Coordinates: 341519..342046 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: G6Z93_RS01525
• Protein ID: WP_001277108.1
• Coordinates: 341246..341512 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z93_RS01505	336904..337572	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
G6Z93_RS01510	337565..338623	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
G6Z93_RS01515	338868..339722	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
G6Z93_RS01520	339993..341096	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
G6Z93_RS01525	341246..341512	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
G6Z93_RS01530	341519..342046	+	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
G6Z93_RS01535	342043..342426	-	384	WP_164476370.1	aspartate 1-decarboxylase autocleavage activator PanM	-
G6Z93_RS01540	342850..343959	+	1110	WP_069913715.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
G6Z93_RS01545	344007..344933	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
G6Z93_RS01550	344930..346207	+	1278	WP_069913714.1	branched chain amino acid ABC transporter permease LivM	-
G6Z93_RS01555	346204..346971	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T151019 WP_000342448.1 NZ_CP049204:341519-342046 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T151019 NZ_CP065177:c2837035-2836682 [Pectobacterium quasiaquaticum]
GTGTGGAAAATTGAAACGACGGACGTGTTTGATAGCTGGTTCGGCACATTAAATGATAGGGATCGCGCATATGTGCTTGC
AGCACTTATCGTGTTACGGGAAAAAGGCCCAATGCTATCTAGGCCTTATGCTGATACGGTAAAAGGCTCCCAGTATAGCA
ACATGAAAGAATTACGTATTCAGAGCGGAGGGATGCCAATACGGGCATTTTTTGCTTTCGATCCACATCGCACGGGGATC
CTCCTCTGTGCGGGGAATAAGTCCGGCAATGAAAAGCGCTTTTATGATGAGATGATTCCGCTTGCCGATCGGGAATTCAC
AAAGTATCTGAAAAAATCAGAGAAGAGAGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT151019 WP_001277108.1 NZ_CP049204:341246-341512 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT151019 NZ_CP065177:c2836677-2836375 [Pectobacterium quasiaquaticum]
ATGGGCAGAACGCTGGAACAGCTCCTTGCGGATGAAAAACCAGACGTCGTCGTTGAGGCTCACGCTATAGCGGCAGACAT
GTTACTCAATATTCATCTCGCCGAACTGCGTGAGAGGGTGCAAAAAACGCAAGTGGAAATGGCGCAGGTGCTTGGATTAA
AACAGCCAACCATTGCGGGAATGGAGAAACCCGGCCGCGATTTGAAACTTTCCACACTCAAACGCTATGTTGAGGCGACC
GGCAGTAAACTGCGCCTTGATGTTGAACTGCCGGACGGTTCTCACTACGGGTTCACGCTGTAA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• PDB: 6GTS
• PDB: 6AJN
• PDB: 6GTQ
• PDB: 6GTO
• PDB: 6GTR
• PDB: 6AJM
• AlphaFold DB: A0A829CN24