Detailed information of TA system TA151012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 101670..102445
• Replicon: plasmid pIncFIB
• Accession: NZ_CP049202
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A0C2EI97
• Locus tag: G6Z99_RS26610
• Protein ID: WP_000405250.1
• Coordinates: 101670..102152 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: G6Z99_RS26615
• Protein ID: WP_072146426.1
• Coordinates: 102143..102445 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS26570	97260..97769	-	510	Protein_105	hypothetical protein	-
G6Z99_RS26575	97847..98137	-	291	WP_158179414.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G6Z99_RS26580	98127..98381	-	255	WP_097468987.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
G6Z99_RS26590	99259..100281	-	1023	WP_164476302.1	replication initiation protein	-
G6Z99_RS26595	100271..100804	-	534	WP_040089446.1	replication protein	-
G6Z99_RS26600	100797..100871	-	75	WP_001367777.1	RepA leader peptide Tap	-
G6Z99_RS26605	101105..101362	-	258	WP_001136637.1	replication regulatory protein RepA	-
G6Z99_RS26610	101670..102152	-	483	WP_000405250.1	GNAT family N-acetyltransferase	Toxin
G6Z99_RS26615	102143..102445	-	303	WP_072146426.1	DUF1778 domain-containing protein	Antitoxin
G6Z99_RS26620	102766..102972	-	207	WP_206438902.1	Hok/Gef family protein	-
-	102959..103020	+	62	NuclAT_1	-	-
-	102959..103020	+	62	NuclAT_1	-	-
-	102959..103020	+	62	NuclAT_1	-	-
-	102959..103020	+	62	NuclAT_1	-	-
G6Z99_RS26625	103261..103470	-	210	WP_040089443.1	hemolysin expression modulator Hha	-
G6Z99_RS26630	103527..103763	-	237	WP_061358569.1	hypothetical protein	-
G6Z99_RS26635	104010..104570	-	561	WP_164476304.1	fertility inhibition protein FinO	-
G6Z99_RS26640	104625..105371	-	747	WP_164476305.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA	1..167766	167766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17649.42 Da
• Isoelectric Point: 7.0386

>T151012 WP_000405250.1 NZ_CP049202:c102152-101670 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEGTLRVVGFYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNVAEQVGVKAVMVHAIDDDARAFYERFGFVQSVVAPDTLFYKV

Nucleotide

• Length: 483 bp

>T151012 NZ_CP065177:c170182-170003 [Pectobacterium quasiaquaticum]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCATCCTAGTAAACTGGGGTTAGTCACAATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAACATCA
GGAAACAAGCAGGAATATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11630.17 Da
• Isoelectric Point: 4.7174

>AT151012 WP_072146426.1 NZ_CP049202:c102445-102143 [Escherichia coli]
IEVFMSTAASVRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT151012 NZ_CP065177:c169975-169565 [Pectobacterium quasiaquaticum]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACAGTGCCAGATCTACCGGGCTGTTT
TTCCGCAGGCGATACGCTAGACGATGCCATCGCGAATGCGAAAGAGGCGATTACCGGACATATCGAACTGCTGATCGAGA
TGGGTCAGGATATTCCTACCGTGTCTTCAGTAGGCCAATGGGCAAAAAGCGCAGAATACGCTGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGCGGTTCAGAAAAAATTAACGTCACGCTGCCTAAATCCCTGATTGACCGAAT
TGATCGTTGCGTGGCGAGTAATCCTGAATTTAAAAGCCGCTCAGGTTTTCTCGCGCAGGCGGCATTAGCGCGAATCTCGT
CTTCCCGATGA

Structures

Toxin

• AlphaFold DB: A0A0C2EI97