Detailed information of TA system TA151011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 97847..98381
• Replicon: plasmid pIncFIB
• Accession: NZ_CP049202
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: G6Z99_RS26575
• Protein ID: WP_158179414.1
• Coordinates: 97847..98137 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G6Z99_RS26580
• Protein ID: WP_097468987.1
• Coordinates: 98127..98381 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS26545	93575..93805	-	231	Protein_100	colicin immunity protein Cui	-
G6Z99_RS26550	93899..94489	+	591	WP_087901131.1	plasmid pRiA4b ORF-3 family protein	-
G6Z99_RS26555	94625..95197	+	573	WP_052939164.1	recombinase family protein	-
G6Z99_RS26560	95232..96635	+	1404	WP_164476301.1	ISKra4 family transposase	-
G6Z99_RS26565	96665..96877	+	213	Protein_104	Tn3 family transposase	-
G6Z99_RS26570	97260..97769	-	510	Protein_105	hypothetical protein	-
G6Z99_RS26575	97847..98137	-	291	WP_158179414.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6Z99_RS26580	98127..98381	-	255	WP_097468987.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
G6Z99_RS26590	99259..100281	-	1023	WP_164476302.1	replication initiation protein	-
G6Z99_RS26595	100271..100804	-	534	WP_040089446.1	replication protein	-
G6Z99_RS26600	100797..100871	-	75	WP_001367777.1	RepA leader peptide Tap	-
G6Z99_RS26605	101105..101362	-	258	WP_001136637.1	replication regulatory protein RepA	-
G6Z99_RS26610	101670..102152	-	483	WP_000405250.1	GNAT family N-acetyltransferase	-
G6Z99_RS26615	102143..102445	-	303	WP_072146426.1	DUF1778 domain-containing protein	-
G6Z99_RS26620	102766..102972	-	207	WP_206438902.1	Hok/Gef family protein	-
-	102959..103020	+	62	NuclAT_1	-	-
-	102959..103020	+	62	NuclAT_1	-	-
-	102959..103020	+	62	NuclAT_1	-	-
-	102959..103020	+	62	NuclAT_1	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA	1..167766	167766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-73)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11371.35 Da
• Isoelectric Point: 10.4715

>T151011 WP_158179414.1 NZ_CP049202:c98137-97847 [Escherichia coli]
MTFNIDFDERALKEWNKLDKTIRDQFKKKLRKLQHNPYTGAARLHGDLAGCFKIKLRASGFRLIYKVIDEEIVIWVIAVG
KRERCNVYNIASERMK

Nucleotide

• Length: 291 bp

>T151011 NZ_CP065177:c25012-24626 [Pectobacterium quasiaquaticum]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCACTATTCGATACGTGGTTTGATGAGCAAACTGAAACGGT
ACAGGAAGAAATTTTGGCACACCTCTCTATACTCGAAGAGGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCAAAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTAGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAC
CGGACACGTAAAGCGATTGTTTTATGTGCAGGAGATAAGAAAGGCGAAGATGAAAAGTTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTGCATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACGCTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9897.24 Da
• Isoelectric Point: 4.5498

>AT151011 WP_097468987.1 NZ_CP049202:c98381-98127 [Escherichia coli]
MSTVQIRVDEELKKNAWQVFEKLNLSPSEALRLFLRYVAENEKLPFNEVSVVVSDNDEDEDILNIVRERLKNPARRIRVN
IDDL

Nucleotide

• Length: 255 bp

>AT151011 NZ_CP065177:c24639-24340 [Pectobacterium quasiaquaticum]
ATGGCGACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAATTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCGTTAGCACTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAAAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCTCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA