Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4908097..4908715 Replicon chromosome
Accession NZ_CP049201
Organism Escherichia coli strain PapRG-04-4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G6Z99_RS23720 Protein ID WP_001291435.1
Coordinates 4908097..4908315 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G6Z99_RS23725 Protein ID WP_000344800.1
Coordinates 4908341..4908715 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Z99_RS23685 4903386..4903958 + 573 WP_000779831.1 YbaY family lipoprotein -
G6Z99_RS23690 4903989..4904300 - 312 WP_000409909.1 MGMT family protein -
G6Z99_RS23700 4904679..4905032 + 354 WP_000878140.1 DUF1428 domain-containing protein -
G6Z99_RS23705 4905074..4906624 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G6Z99_RS23710 4906788..4907258 - 471 WP_000136192.1 YlaC family protein -
G6Z99_RS23715 4907374..4907925 - 552 WP_000102564.1 maltose O-acetyltransferase -
G6Z99_RS23720 4908097..4908315 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
G6Z99_RS23725 4908341..4908715 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G6Z99_RS23730 4909261..4912410 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
G6Z99_RS23735 4912433..4913626 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T151010 WP_001291435.1 NZ_CP049201:c4908315-4908097 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T151010 NZ_CP065176:26490-26795 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT151010 WP_000344800.1 NZ_CP049201:c4908715-4908341 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT151010 NZ_CP065176:26270-26488 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References