Detailed information of TA system TA151007

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4593583..4594370
• Replicon: chromosome
• Accession: NZ_CP049201
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: G6Z99_RS22090
• Protein ID: WP_164476064.1
• Coordinates: 4593583..4593960 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2H9G3E7
• Locus tag: G6Z99_RS22095
• Protein ID: WP_000066236.1
• Coordinates: 4594011..4594370 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS22050	4588822..4589577	-	756	WP_164476062.1	hydroxyacylglutathione hydrolase	-
G6Z99_RS22055	4589611..4590333	+	723	WP_021556435.1	class I SAM-dependent methyltransferase	-
G6Z99_RS22060	4590330..4590797	-	468	WP_021556436.1	ribonuclease HI	-
G6Z99_RS22065	4590862..4591593	+	732	WP_001382826.1	DNA polymerase III subunit epsilon	-
G6Z99_RS22075	4591935..4592779	-	845	Protein_4319	DUF4942 domain-containing protein	-
G6Z99_RS22080	4592860..4593063	-	204	WP_164476063.1	DUF957 domain-containing protein	-
G6Z99_RS22085	4593095..4593586	-	492	WP_000777682.1	hypothetical protein	-
G6Z99_RS22090	4593583..4593960	-	378	WP_164476064.1	TA system toxin CbtA family protein	Toxin
G6Z99_RS22095	4594011..4594370	-	360	WP_000066236.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G6Z99_RS22100	4594394..4594615	-	222	WP_000691981.1	DUF987 domain-containing protein	-
G6Z99_RS22105	4594636..4595115	-	480	WP_000437750.1	DNA repair protein RadC	-
G6Z99_RS22110	4595127..4595597	-	471	WP_000131762.1	antirestriction protein	-
G6Z99_RS22115	4595865..4596683	-	819	WP_164476065.1	DUF945 domain-containing protein	-
G6Z99_RS22120	4596803..4597036	-	234	WP_001614358.1	DUF905 domain-containing protein	-
G6Z99_RS22125	4597113..4597565	-	453	WP_001061894.1	hypothetical protein	-
G6Z99_RS22130	4597625..4598065	-	441	Protein_4330	DUF4339 domain-containing protein	-
G6Z99_RS22135	4598116..4598691	-	576	WP_164476066.1	hypothetical protein	-
G6Z99_RS22140	4598789..4598983	-	195	Protein_4332	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14150.94 Da
• Isoelectric Point: 7.4209

>T151007 WP_164476064.1 NZ_CP049201:c4593960-4593583 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMARNDYRTVTDITTGKYRGGHR

Nucleotide

• Length: 378 bp

>T151007 NZ_CP065172:c5198163-5197780 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGCCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13127.02 Da
• Isoelectric Point: 7.0261

>AT151007 WP_000066236.1 NZ_CP049201:c4594370-4594011 [Escherichia coli]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 360 bp

>AT151007 NZ_CP065172:c5198405-5198163 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A2H9G3E7