Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4162250..4162845 Replicon chromosome
Accession NZ_CP049201
Organism Escherichia coli strain PapRG-04-4

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag G6Z99_RS20135 Protein ID WP_000239579.1
Coordinates 4162495..4162845 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag G6Z99_RS20130 Protein ID WP_001223208.1
Coordinates 4162250..4162501 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6Z99_RS20120 4157915..4161694 + 3780 WP_164475998.1 autotransporter assembly complex protein TamB -
G6Z99_RS20125 4161697..4162038 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
G6Z99_RS20130 4162250..4162501 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
G6Z99_RS20135 4162495..4162845 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
G6Z99_RS20140 4162925..4163455 - 531 WP_000055075.1 inorganic diphosphatase -
G6Z99_RS20145 4163765..4164721 + 957 WP_000265942.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
G6Z99_RS20150 4164861..4166363 + 1503 WP_164475999.1 sugar ABC transporter ATP-binding protein -
G6Z99_RS20155 4166377..4167399 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T151003 WP_000239579.1 NZ_CP049201:4162495-4162845 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T151003 NZ_CP065172:4016228-4016446 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT151003 WP_001223208.1 NZ_CP049201:4162250-4162501 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT151003 NZ_CP065172:4015827-4016201 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAACTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References