Detailed information of TA system TA151001

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3437571..3438406
• Replicon: chromosome
• Accession: NZ_CP049201
• Organism: Escherichia coli strain PapRG-04-4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: G6Z99_RS16700
• Protein ID: WP_158181347.1
• Coordinates: 3438029..3438406 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: G6Z99_RS16695
• Protein ID: WP_158181348.1
• Coordinates: 3437571..3437939 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z99_RS16660	3433300..3434184	+	885	WP_158181352.1	50S ribosome-binding GTPase	-
G6Z99_RS16665	3434303..3434980	+	678	WP_164475882.1	hypothetical protein	-
G6Z99_RS16670	3434986..3435219	+	234	WP_001278287.1	DUF905 family protein	-
G6Z99_RS16675	3435318..3436136	+	819	WP_164475883.1	DUF945 domain-containing protein	-
G6Z99_RS16680	3436228..3436713	+	486	WP_041124170.1	antirestriction protein	-
G6Z99_RS16685	3436729..3437205	+	477	WP_041124171.1	RadC family protein	-
G6Z99_RS16690	3437274..3437495	+	222	WP_000692307.1	DUF987 domain-containing protein	-
G6Z99_RS16695	3437571..3437939	+	369	WP_158181348.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
G6Z99_RS16700	3438029..3438406	+	378	WP_158181347.1	TA system toxin CbtA family protein	Toxin
G6Z99_RS16705	3438403..3438891	+	489	WP_164475884.1	hypothetical protein	-
G6Z99_RS16710	3438903..3439100	+	198	WP_000839242.1	DUF957 domain-containing protein	-
G6Z99_RS16715	3439185..3440027	+	843	WP_158181363.1	DUF4942 domain-containing protein	-
G6Z99_RS16720	3440265..3440393	+	129	Protein_3286	virulence RhuM family protein	-
G6Z99_RS16725	3440822..3442006	+	1185	WP_164475885.1	sugar efflux transporter	-
G6Z99_RS16730	3442117..3443040	+	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3421528..3451628	30100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14148.09 Da
• Isoelectric Point: 9.1660

>T151001 WP_158181347.1 NZ_CP049201:3438029-3438406 [Escherichia coli]
MNTLPDTHVRGASRRPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T151001 NZ_CP065172:2415845-2416162 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13576.37 Da
• Isoelectric Point: 6.4757

>AT151001 WP_158181348.1 NZ_CP049201:3437571-3437939 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT151001 NZ_CP065172:2416238-2416534 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCCGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCAGCCAACCGGTGCAGCCAGAAAA
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA